Ab107980

antibody from Aladdin Scientific

Targeting: H3C4 H3/b, H3FB, HIST1H3D

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

Ab107980

Provider

Aladdin Scientific

Product name

Recombinant Histone H3 Antibody

Antibody type

Recombinant

Description

Rabbit anti Human Histone H3 Antibody, Recombinant (RR692), could be used for WB, IHC, Flow, IP and so on.Application:WB: 1/1000 - 1/5000 IHC: 1/800 - 1/1600Flow: 1/50 - 1/200IP: 1/100

Reactivity

Human, Mouse, Rat, Bovine, Canine, Zebrafish

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

AA 1-100


Antibody clone number

RR692

Vial size

100_l,10_l,1ml,50_l

Concentration

1 mg/ml

Storage

Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Western blot

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Recombinant Histone H3 Antibody (Ab107980) - Western Blot All lanes: Recombinant Histone H3 Antibody (Ab107980) at 1/3000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution Predicted band size: 15 kDa Observed band size: 15, 17 kDa Exposure time: 7.1 s

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Recombinant Histone H3 Antibody (Ab107980) - Western Blot All lanes: Recombinant Histone H3 Antibody (Ab107980) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution Predicted band size: 15 kDa Observed band size: 15, 17 kDa Exposure time: 33.2 s

Immunohistochemistry

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Recombinant Histone H3 Antibody (Ab107980) - IHC Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrial cancer tissue labelling Histone H3 with Recombinant Histone H3 Antibody (Ab107980) at 1/800. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.

Flow cytometry

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Recombinant Histone H3 Antibody (Ab107980) - Flow Cytometry Overlay histogram showing HeLa cells stained with Recombinant Histone H3 Antibody (Ab107980) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in Recombinant Histone H3 Antibody (Ab107980) (1/200 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.