PA5-101017

antibody from Invitrogen Antibodies

Targeting: H1-3 H1.3, H1d, H1F3, H1s-2, HIST1H1D

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-101017

Provider

Invitrogen Antibodies

Product name

Phospho-Histone H1.3 (Thr18) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Antibody detects endogenous levels of Histone H1.3 only when phosphorylated at Thr18.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-Histone H1.3/H1.4 (Thr17) in 293 cell lysate. Samples were incubated with Phospho-Histone H1.3/H1.4 (Thr17) polyclonal antibody (Product # PA5-101017).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Phospho-Histone H1.3/H1.4 (Thr17) in HepG2. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with Phospho-Histone H1.3/H1.4 (Thr17) polyclonal antibody (Product # PA5-101017) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of paraffin-embedded Phospho-Histone H1.3/H1.4 (Thr17) in mouse kidney tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-Histone H1.3/H1.4 (Thr17) polyclonal antibody (Product # PA5-101017) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.