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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-117470 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Histone H1.3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody has been tested in direct-ELISA
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.11 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Histone H1.3 using Histone H1.3 Polyclonal Antibody (Product # PA5-117470) at 1.5 µg/mL. Positive WB detected in: PC-3 whole cell lysate, Rat spleen tissue, Mouse spleen tissue. Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 23 kDa. Observed band size: 23 kDa
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of Histone H1.3 in HeLa cells using Histone H1.3 Polyclonal Antibody (Product # PA5-117470) at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Histone H1.3 in paraffin-embedded human ovarian cancer using Histone H1.3 Polyclonal Antibody (Product # PA5-117470) diluted at 1:100.
