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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Chromatin Immunoprecipitation [2]
- Other assay [1]
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- Product number
- 39120 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Histone H2BK120ac Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 10 µL
- Concentration
- Conc. Not Determined
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Histone H2B acetyl Lys120 pAb tested by Western blot. HeLa acid extract (5 µg per lane) was probed with Histone H2B acetyl Lys120 polyclonal antibody (1:2,000 dilution). Lane 1: Human recombinant H2B (200 ng). Lane 2: No treatment. Lane 3: Cells treated with sodium butyrate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of H2BK120ac by immunofluorescence U2OS cells were stained with H2BK120ac antibody at a dilution of 1:500. Left panel: DAPI. Middle panel: H2BK120ac antibody staining. Right panel: merge.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Histone H2B acetyl Lys120 pAb tested by ChIP analysis. Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 10 µl of Histone H2B acetyl Lys120 pAb or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the indicated gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Histone H2B acetyl Lys120 pAb tested by ChIP-Seq. ChIP was performed using chromatin from the H1 human embryonic stem cell line. ChIP DNA was sequenced on the Illumina GA II and sequence tags were mapped to identify H2BK120Ac binding. The image shows H2BK120Ac binding in and around the IGSF21 gene on chromosome 1.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Histone H2B acetyl Lys120 pAb tested by dot blot analysis. Specificity Data: Dot blot analysis was used to confirm the specificity of Histone H2B acetyl Lys120 pAb for acetyl-Lys 120 of histone H2B. Decreasing amounts of modified and unmodified peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:2,000. Lane 1: Peptide acetylated at lysine 5 of H2B. Lane 2: Unmodified lysine 5 peptide. Lane 3: Peptide acetylated at lysine 16 of H2B. Lane 4: Unmodified lysine 16 peptide. Lane 5: Peptide acetylated at lysine 120 of H2B. Lane 6: Unmodified lysine 120 peptide.
