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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PA5-24220 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HIST1H2BJ Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, chicken, mouse, non-human primate, rat and Xenopus and zebrafish based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.4 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a HIST1H2BJ polyclonal antibody (Product # PA5-24220) in CEM, HL-60, NCI-H460 cell lysates (35 µg per lane).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Histone H2BJ was performed by loading 20 µg of Nuclear protein extracts from BEAS-2B (lane 1), and 150 ng of Purified Histones containing H2BJ (lane 2) and Histone Octamer containing canonical H2B (lane 3) in sample buffer and 5 µL Spectra Multicolor Broad Range Protein Ladder (Product # 26634) onto a 15% polyacrylamide gel. Proteins were transferred to PVDF membrane (Product # 88518) at 65V for 90 min on ice. Membrane was blocked in 5% Milk in PBST for 1 hour at room temperature. Histone H2BJ was detected at approximately 15 kDa using a Histone H2BJ polyclonal antibody (Product # PA5-24220) at a dilution of 1:500 in 0.5% Milk in PBST overnight at 4ºC. Followed by goat anti-rabbit alkaline phosphatase secondary antibody at a dilution of 1:20,000. Chemiluminescent detection was performed using ECF substrate. Data courtesy of the laboratory of Dr. Yvonne Fondufe-Mittendorf.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human hepatocarcinoma using a HIST1H2BJ polyclonal antibody (Product # PA5-24220), followed by HRP-conjugated secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of CEM cells using a HIST1H2BJ polyclonal antibody (Product # PA5-24220) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
