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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
- Flow cytometry [2]
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- Product number
- PA5-24220 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HIST1H2BJ Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, chicken, mouse, non-human primate, rat and Xenopus and zebrafish based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.4 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of HIST1H2BJ in formalin fixed and paraffin embedded human hepatocarcinoma. Samples were incubated with HIST1H2BJ polyclonal antibody (Product # PA5-24220) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of CEM cells using a HIST1H2BJ polyclonal antibody (Product # PA5-24220) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of HIST1H2BJ in CEM cells (right histogram). Samples were incubated with HIST1H2BJ polyclonal antibody (Product # PA5-24220) followed by FITC-conjugated goat-anti-rabbit secondary antibody. Negative control cell (left histogram).
