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Learn500-P241-1MG
antibody from Invitrogen Antibodies
Targeting: DEFB103A
DEFB-3, DEFB103, DEFB3, HBD-3, HBD3, HBP-3, HBP3
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- ELISA [2]
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- Product number
- 500-P241-1MG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- beta-3 Defensin Polyclonal Antibody, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: GIINTLQKYY CRVRGGRCAV LSCLPKEEQI GKCSTRGRKC CRRKK. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human BD-3. Anti-Human BD-3-specific antibody was purified by affinity chromatography employing an immobilized Human BD-3 matrix. Sandwich ELISA: To detect Human BD-3 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human BD-3 (500-P241Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human BD-3. Western Blot: To detect Human BD-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human BD-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 1 mg
- Concentration
- 0.1-1.0 mg/mL
- Storage
- -20°C
Submitted references Human beta-defensin 3 contributes to the carcinogenesis of cervical cancer via activation of NF-κB signaling.
Bitter and sweet taste receptors regulate human upper respiratory innate immunity.
IL-1F5, -F6, -F8, and -F9: a novel IL-1 family signaling system that is active in psoriasis and promotes keratinocyte antimicrobial peptide expression.
An antimicrobial peptide regulates tumor-associated macrophage trafficking via the chemokine receptor CCR2, a model for tumorigenesis.
UV-B radiation induces the expression of antimicrobial peptides in human keratinocytes in vitro and in vivo.
Association of a genetic polymorphism (-44 C/G SNP) in the human DEFB1 gene with expression and inducibility of multiple beta-defensins in gingival keratinocytes.
Quantification of human beta-defensin-2 and -3 in body fluids: application for studies of innate immunity.
Xu D, Zhang B, Liao C, Zhang W, Wang W, Chang Y, Shao Y
Oncotarget 2016 Nov 15;7(46):75902-75913
Oncotarget 2016 Nov 15;7(46):75902-75913
Bitter and sweet taste receptors regulate human upper respiratory innate immunity.
Lee RJ, Kofonow JM, Rosen PL, Siebert AP, Chen B, Doghramji L, Xiong G, Adappa ND, Palmer JN, Kennedy DW, Kreindler JL, Margolskee RF, Cohen NA
The Journal of clinical investigation 2014 Mar;124(3):1393-405
The Journal of clinical investigation 2014 Mar;124(3):1393-405
IL-1F5, -F6, -F8, and -F9: a novel IL-1 family signaling system that is active in psoriasis and promotes keratinocyte antimicrobial peptide expression.
Johnston A, Xing X, Guzman AM, Riblett M, Loyd CM, Ward NL, Wohn C, Prens EP, Wang F, Maier LE, Kang S, Voorhees JJ, Elder JT, Gudjonsson JE
Journal of immunology (Baltimore, Md. : 1950) 2011 Feb 15;186(4):2613-22
Journal of immunology (Baltimore, Md. : 1950) 2011 Feb 15;186(4):2613-22
An antimicrobial peptide regulates tumor-associated macrophage trafficking via the chemokine receptor CCR2, a model for tumorigenesis.
Jin G, Kawsar HI, Hirsch SA, Zeng C, Jia X, Feng Z, Ghosh SK, Zheng QY, Zhou A, McIntyre TM, Weinberg A
PloS one 2010 Jun 8;5(6):e10993
PloS one 2010 Jun 8;5(6):e10993
UV-B radiation induces the expression of antimicrobial peptides in human keratinocytes in vitro and in vivo.
Gläser R, Navid F, Schuller W, Jantschitsch C, Harder J, Schröder JM, Schwarz A, Schwarz T
The Journal of allergy and clinical immunology 2009 May;123(5):1117-23
The Journal of allergy and clinical immunology 2009 May;123(5):1117-23
Association of a genetic polymorphism (-44 C/G SNP) in the human DEFB1 gene with expression and inducibility of multiple beta-defensins in gingival keratinocytes.
Kalus AA, Fredericks LP, Hacker BM, Dommisch H, Presland RB, Kimball JR, Dale BA
BMC oral health 2009 Aug 27;9:21
BMC oral health 2009 Aug 27;9:21
Quantification of human beta-defensin-2 and -3 in body fluids: application for studies of innate immunity.
Ghosh SK, Gerken TA, Schneider KM, Feng Z, McCormick TS, Weinberg A
Clinical chemistry 2007 Apr;53(4):757-65
Clinical chemistry 2007 Apr;53(4):757-65
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA: To detect Human BD-3 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of beta-3 Defensin Polyclonal Antibody (Product # 500-P241-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech beta-3 Defensin Polyclonal Antibody, Biotin (Product # 500-P241BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human BD-3.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA: To detect Human BD-3 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of beta-3 Defensin Polyclonal Antibody (Product # 500-P241-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech beta-3 Defensin Polyclonal Antibody, Biotin (Product # 500-P241BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human BD-3.
