MA3-003

antibody from Invitrogen Antibodies

Targeting: BCAP31 6C6-Ag, BAP31, CDM, DXS1357E

Immunoprecipitation

Flow cytometry

Antibody data

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Validation data

Product number: MA3-003 - Provider product page
Provider: Invitrogen Antibodies
Product name: BAP31 Monoclonal Antibody (CC-4)
Antibody type: Monoclonal
Antigen: Other
Description: MA3-003 detects BAP31 from human, non-human primate, bovine and hamster samples. MA3-003 has been successfully used in Western blot, immunofluorescent, and immunohistochemical procedures. By Western blot, this antibody detects a 28-kDa protein corresponding to human BAP31. The MA3-003 antigen is solubilized protein from human neuroendocrine cell line IMR-32, followed by an extract of the small lung human carcinoma cell line SCC-9. MA3-003 binds to a more proximal region of BAP31 (amino acids 123-229). This sequence is conserved in non-human primate, bovine and hamster species.
Reactivity: Human, Bovine, Hamster
Host: Rat
Isotype: IgG
Antibody clone number: CC-4
Vial size: 100 µL
Concentration: Conc. Not Determined
Storage: -20° C, Avoid Freeze/Thaw Cycles

BCAP31 protein structure - MA3-003 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on membrane enriched extracts (30µg lysate) of COS-7 (Lane 1), Raji (Lane 2), Molt-4 (Lane 3), HeLa (Lane 4), MCF7 (Lane 5), K-562 (Lane6), Hep G2 (Lane 7), Jurkat (Lane 8), HT-1080 (Lane 9) and HEK-293 (Lane 10). The blots were probed with anti-BAP31 Rat Monoclonal Antibody (Product # MA3-003, 1:1500 dilution) and detected by chemiluminescence using Goat anti-Rat IgG2a Secondary Antibody, HRP conjugate (Product # PA1-84709, 0.4µg/mL, 1:2500 dilution). A 28 kDa band corresponding to BAP31 was observed across tissues tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of BAP31 was achieved by transfecting HeLa cells with BAP31 specific siRNAs (Silencer® select Product # s19722, s19721). Western blot analysis (Fig a) was performed using membrane enriched extracts from the BAP31 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with BAP31 Antibody (Product # MA3-003, 1:1500 dilution) and Rabbit anti-Rat IgG (H+L) Secondary Antibody, HRP conjugate ( Product # PA1-29927, 0.25µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to BAP31.