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Western blot
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA5-29986 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Centrin 1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Jurkat, 293T, A431. Predicted reactivity: Mouse (95%), Rat (95%), Xenopus laevis (84%), Chicken (84%), Bovine (92%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Bovine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Centrin 1 was performed in A431 cells fixed in ice cold MeOH for 5 min. Green: Centrin 1 Polyclonal Antibody (Product # PA5 29986) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Centrin-1 protein from 293T whole cell extracts using 5 µg of Centrin-1 antibody [N1C3] (Product # PA5-29986). Western blot analysis was performed using Centrin-1 antibody [N1C3] (Product # PA5-29986) diluted at 1:500.
