Antibody data
- Antibody Data
- Antigen structure
- References [35]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 12794-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#12794-1-AP, RRID:AB_2077371
- Product name
- Centrin 1 antibody
- Antibody type
- Polyclonal
- Description
- Centrin 1 antibody (Cat. #12794-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IF, WB,ELISA.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Uncovering structural themes across cilia microtubule inner proteins with implications for human cilia function.
Hornerin mediates phosphorylation of the polo-box domain in Plk1 by Chk1 to induce death in mitosis.
K-Ras Binds Calmodulin-Related Centrin1 with Potential Implications for K-Ras Driven Cancer Cell Stemness.
Absence of CEP78 causes photoreceptor and sperm flagella impairments in mice and a human individual.
CDK2 inhibition disorders centrosome stoichiometry and alters cellular outcomes in aneuploid cancer cells.
Tau tubulin kinase 1 and 2 regulate ciliogenesis and human pluripotent stem cells-derived neural rosettes.
CCHCR1-astrin interaction promotes centriole duplication through recruitment of CEP72.
ENKD1 promotes CP110 removal through competing with CEP97 to initiate ciliogenesis.
Molecular mechanisms underlying the role of the centriolar CEP164-TTBK2 complex in ciliopathies.
Cartwheel disassembly regulated by CDK1-cyclin B kinase allows human centriole disengagement and licensing.
Loss of DRC1 function leads to multiple morphological abnormalities of the sperm flagella and male infertility in human and mouse.
PLK4-phosphorylated NEDD1 facilitates cartwheel assembly and centriole biogenesis initiations.
O-GlcNAc transferase regulates centriole behavior and intraflagellar transport to promote ciliogenesis.
ASK1-Mediated Phosphorylation Blocks HDAC6 Ubiquitination and Degradation to Drive the Disassembly of Photoreceptor Connecting Cilia.
Homozygous mutations in DZIP1 can induce asthenoteratospermia with severe MMAF.
Secreted Wnt6 mediates diabetes-associated centrosome amplification via its receptor FZD4.
Characterisation of centriole biogenesis during multiciliation in planarians.
Centrosomal Localization of RXRα Promotes PLK1 Activation and Mitotic Progression and Constitutes a Tumor Vulnerability.
Posterior Neocortex-Specific Regulation of Neuronal Migration by CEP85L Identifies Maternal Centriole-Dependent Activation of CDK5.
Wdr47 Controls Neuronal Polarization through the Camsap Family Microtubule Minus-End-Binding Proteins.
Bisphenol A disrupts mitotic progression via disturbing spindle attachment to kinetochore and centriole duplication in cancer cell lines.
MICAL-L1 coordinates ciliogenesis by recruiting EHD1 to the primary cilium.
Resting cells rely on the DNA helicase component MCM2 to build cilia.
c-Cbl Acts as an E3 Ligase Against DDA3 for Spindle Dynamics and Centriole Duplication during Mitosis.
Parental centrioles are dispensable for deuterosome formation and function during basal body amplification.
Vesicular trafficking plays a role in centriole disengagement and duplication.
PEDF regulates plasticity of a novel lipid-MTOC axis in prostate cancer-associated fibroblasts.
Cytoplasmic E2f4 forms organizing centres for initiation of centriole amplification during multiciliogenesis.
DNA replication licensing factor Cdc6 and Plk4 kinase antagonistically regulate centrosome duplication via Sas-6.
NudC regulates actin dynamics and ciliogenesis by stabilizing cofilin 1.
The tumor suppressor proteins ASPP1 and ASPP2 interact with C-Nap1 and regulate centrosome linker reassembly.
Centriolar satellites assemble centrosomal microcephaly proteins to recruit CDK2 and promote centriole duplication.
Kif3a interacts with Dynactin subunit p150 Glued to organize centriole subdistal appendages.
The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis.
Misfolded Gβ is recruited to cytoplasmic dynein by Nudel for efficient clearance.
Andersen JS, Vijayakumaran A, Godbehere C, Lorentzen E, Mennella V, Schou KB
Nature communications 2024 Mar 27;15(1):2687
Nature communications 2024 Mar 27;15(1):2687
Hornerin mediates phosphorylation of the polo-box domain in Plk1 by Chk1 to induce death in mitosis.
Song H, Kim EH, Hong J, Gwon D, Kim JW, Bae GU, Jang CY
Cell death and differentiation 2023 Sep;30(9):2151-2166
Cell death and differentiation 2023 Sep;30(9):2151-2166
K-Ras Binds Calmodulin-Related Centrin1 with Potential Implications for K-Ras Driven Cancer Cell Stemness.
Manoharan GB, Laurini C, Bottone S, Ben Fredj N, Abankwa DK
Cancers 2023 Jun 7;15(12)
Cancers 2023 Jun 7;15(12)
Absence of CEP78 causes photoreceptor and sperm flagella impairments in mice and a human individual.
Zhu T, Zhang Y, Sheng X, Zhang X, Chen Y, Zhu H, Guo Y, Qi Y, Zhao Y, Zhou Q, Chen X, Guo X, Zhao C
eLife 2023 Feb 9;12
eLife 2023 Feb 9;12
CDK2 inhibition disorders centrosome stoichiometry and alters cellular outcomes in aneuploid cancer cells.
Chen Z, Liu X, Kawakami M, Liu X, Baker A, Bhatawadekar A, Tyutyunyk-Massey L, Narayan K, Dmitrovsky E
Cancer biology & therapy 2023 Dec 31;24(1):2279241
Cancer biology & therapy 2023 Dec 31;24(1):2279241
Tau tubulin kinase 1 and 2 regulate ciliogenesis and human pluripotent stem cells-derived neural rosettes.
Binó L, Čajánek L
Scientific reports 2023 Aug 9;13(1):12884
Scientific reports 2023 Aug 9;13(1):12884
CCHCR1-astrin interaction promotes centriole duplication through recruitment of CEP72.
Ying Z, Wang K, Wu J, Wang M, Yang J, Wang X, Zhou G, Chen H, Xu H, Sze SCW, Gao F, Li C, Sha O
BMC biology 2022 Oct 24;20(1):240
BMC biology 2022 Oct 24;20(1):240
ENKD1 promotes CP110 removal through competing with CEP97 to initiate ciliogenesis.
Song T, Yang Y, Zhou P, Ran J, Zhang L, Wu X, Xie W, Zhong T, Liu H, Liu M, Li D, Zhao H, Zhou J
EMBO reports 2022 May 4;23(5):e54090
EMBO reports 2022 May 4;23(5):e54090
Molecular mechanisms underlying the role of the centriolar CEP164-TTBK2 complex in ciliopathies.
Rosa E Silva I, Binó L, Johnson CM, Rutherford TJ, Neuhaus D, Andreeva A, Čajánek L, van Breugel M
Structure (London, England : 1993) 2022 Jan 6;30(1):114-128.e9
Structure (London, England : 1993) 2022 Jan 6;30(1):114-128.e9
Cartwheel disassembly regulated by CDK1-cyclin B kinase allows human centriole disengagement and licensing.
Huang F, Xu X, Xin G, Zhang B, Jiang Q, Zhang C
The Journal of biological chemistry 2022 Dec;298(12):102658
The Journal of biological chemistry 2022 Dec;298(12):102658
Loss of DRC1 function leads to multiple morphological abnormalities of the sperm flagella and male infertility in human and mouse.
Zhang J, He X, Wu H, Zhang X, Yang S, Liu C, Liu S, Hua R, Zhou S, Zhao S, Hu F, Zhang J, Liu W, Cheng H, Gao Y, Zhang F, Cao Y, Liu M
Human molecular genetics 2021 Oct 13;30(21):1996-2011
Human molecular genetics 2021 Oct 13;30(21):1996-2011
PLK4-phosphorylated NEDD1 facilitates cartwheel assembly and centriole biogenesis initiations.
Chi W, Wang G, Xin G, Jiang Q, Zhang C
The Journal of cell biology 2021 Jan 4;220(1)
The Journal of cell biology 2021 Jan 4;220(1)
O-GlcNAc transferase regulates centriole behavior and intraflagellar transport to promote ciliogenesis.
Yu F, Li T, Sui Y, Chen Q, Yang S, Yang J, Hong R, Li D, Yan X, Zhao W, Zhu X, Zhou J
Protein & cell 2020 Nov;11(11):852-857
Protein & cell 2020 Nov;11(11):852-857
ASK1-Mediated Phosphorylation Blocks HDAC6 Ubiquitination and Degradation to Drive the Disassembly of Photoreceptor Connecting Cilia.
Ran J, Liu M, Feng J, Li H, Ma H, Song T, Cao Y, Zhou P, Wu Y, Yang Y, Yang Y, Yu F, Guo H, Zhang L, Xie S, Li D, Gao J, Zhang X, Zhu X, Zhou J
Developmental cell 2020 May 4;53(3):287-299.e5
Developmental cell 2020 May 4;53(3):287-299.e5
Homozygous mutations in DZIP1 can induce asthenoteratospermia with severe MMAF.
Lv M, Liu W, Chi W, Ni X, Wang J, Cheng H, Li WY, Yang S, Wu H, Zhang J, Gao Y, Liu C, Li C, Yang C, Tan Q, Tang D, Zhang J, Song B, Chen YJ, Li Q, Zhong Y, Zhang Z, Saiyin H, Jin L, Xu Y, Zhou P, Wei Z, Zhang C, He X, Zhang F, Cao Y
Journal of medical genetics 2020 Jul;57(7):445-453
Journal of medical genetics 2020 Jul;57(7):445-453
Secreted Wnt6 mediates diabetes-associated centrosome amplification via its receptor FZD4.
He QJ, Wang P, Liu QQ, Wu QG, Li YF, Wang J, Lee SC
American journal of physiology. Cell physiology 2020 Jan 1;318(1):C48-C62
American journal of physiology. Cell physiology 2020 Jan 1;318(1):C48-C62
Characterisation of centriole biogenesis during multiciliation in planarians.
Li Y, Guo F, Jing Q, Zhu X, Yan X
Biology of the cell 2020 Dec;112(12):398-408
Biology of the cell 2020 Dec;112(12):398-408
Centrosomal Localization of RXRα Promotes PLK1 Activation and Mitotic Progression and Constitutes a Tumor Vulnerability.
Xie G, Zhou Y, Tu X, Ye X, Xu L, Xiao Z, Wang Q, Wang X, Du M, Chen Z, Chi X, Zhang X, Xia J, Zhang X, Zhou Y, Li Z, Xie C, Sheng L, Zeng Z, Zhou H, Yin Z, Su Y, Xu Y, Zhang XK
Developmental cell 2020 Dec 21;55(6):707-722.e9
Developmental cell 2020 Dec 21;55(6):707-722.e9
Posterior Neocortex-Specific Regulation of Neuronal Migration by CEP85L Identifies Maternal Centriole-Dependent Activation of CDK5.
Kodani A, Kenny C, Lai A, Gonzalez DM, Stronge E, Sejourne GM, Isacco L, Partlow JN, O'Donnell A, McWalter K, Byrne AB, Barkovich AJ, Yang E, Hill RS, Gawlinski P, Wiszniewski W, Cohen JS, Fatemi SA, Baranano KW, Sahin M, Vossler DG, Yuskaitis CJ, Walsh CA
Neuron 2020 Apr 22;106(2):246-255.e6
Neuron 2020 Apr 22;106(2):246-255.e6
Wdr47 Controls Neuronal Polarization through the Camsap Family Microtubule Minus-End-Binding Proteins.
Chen Y, Zheng J, Li X, Zhu L, Shao Z, Yan X, Zhu X
Cell reports 2020 Apr 21;31(3):107526
Cell reports 2020 Apr 21;31(3):107526
Bisphenol A disrupts mitotic progression via disturbing spindle attachment to kinetochore and centriole duplication in cancer cell lines.
Kim S, Gwon D, Kim JA, Choi H, Jang CY
Toxicology in vitro : an international journal published in association with BIBRA 2019 Sep;59:115-125
Toxicology in vitro : an international journal published in association with BIBRA 2019 Sep;59:115-125
MICAL-L1 coordinates ciliogenesis by recruiting EHD1 to the primary cilium.
Xie S, Farmer T, Naslavsky N, Caplan S
Journal of cell science 2019 Nov 14;132(22)
Journal of cell science 2019 Nov 14;132(22)
Resting cells rely on the DNA helicase component MCM2 to build cilia.
Casar Tena T, Maerz LD, Szafranski K, Groth M, Blätte TJ, Donow C, Matysik S, Walther P, Jeggo PA, Burkhalter MD, Philipp M
Nucleic acids research 2019 Jan 10;47(1):134-151
Nucleic acids research 2019 Jan 10;47(1):134-151
c-Cbl Acts as an E3 Ligase Against DDA3 for Spindle Dynamics and Centriole Duplication during Mitosis.
Gwon D, Hong J, Jang CY
Molecules and cells 2019 Dec 31;42(12):840-849
Molecules and cells 2019 Dec 31;42(12):840-849
Parental centrioles are dispensable for deuterosome formation and function during basal body amplification.
Zhao H, Chen Q, Fang C, Huang Q, Zhou J, Yan X, Zhu X
EMBO reports 2019 Apr;20(4)
EMBO reports 2019 Apr;20(4)
Vesicular trafficking plays a role in centriole disengagement and duplication.
Xie S, Reinecke JB, Farmer T, Bahl K, Yeow I, Nichols BJ, McLamarrah TA, Naslavsky N, Rogers GC, Caplan S
Molecular biology of the cell 2018 Nov 1;29(22):2622-2631
Molecular biology of the cell 2018 Nov 1;29(22):2622-2631
PEDF regulates plasticity of a novel lipid-MTOC axis in prostate cancer-associated fibroblasts.
Nardi F, Fitchev P, Franco OE, Ivanisevic J, Scheibler A, Hayward SW, Brendler CB, Welte MA, Crawford SE
Journal of cell science 2018 Jul 11;131(13)
Journal of cell science 2018 Jul 11;131(13)
Cytoplasmic E2f4 forms organizing centres for initiation of centriole amplification during multiciliogenesis.
Mori M, Hazan R, Danielian PS, Mahoney JE, Li H, Lu J, Miller ES, Zhu X, Lees JA, Cardoso WV
Nature communications 2017 Jul 4;8:15857
Nature communications 2017 Jul 4;8:15857
DNA replication licensing factor Cdc6 and Plk4 kinase antagonistically regulate centrosome duplication via Sas-6.
Xu X, Huang S, Zhang B, Huang F, Chi W, Fu J, Wang G, Li S, Jiang Q, Zhang C
Nature communications 2017 Apr 27;8:15164
Nature communications 2017 Apr 27;8:15164
NudC regulates actin dynamics and ciliogenesis by stabilizing cofilin 1.
Zhang C, Zhang W, Lu Y, Yan X, Yan X, Zhu X, Liu W, Yang Y, Zhou T
Cell research 2016 Feb;26(2):239-53
Cell research 2016 Feb;26(2):239-53
The tumor suppressor proteins ASPP1 and ASPP2 interact with C-Nap1 and regulate centrosome linker reassembly.
Zhang Y, Wang Y, Wei Y, Ma J, Peng J, Wumaier R, Shen S, Zhang P, Yu L
Biochemical and biophysical research communications 2015 Mar 13;458(3):494-500
Biochemical and biophysical research communications 2015 Mar 13;458(3):494-500
Centriolar satellites assemble centrosomal microcephaly proteins to recruit CDK2 and promote centriole duplication.
Kodani A, Yu TW, Johnson JR, Jayaraman D, Johnson TL, Al-Gazali L, Sztriha L, Partlow JN, Kim H, Krup AL, Dammermann A, Krogan NJ, Walsh CA, Reiter JF
eLife 2015 Aug 22;4
eLife 2015 Aug 22;4
Kif3a interacts with Dynactin subunit p150 Glued to organize centriole subdistal appendages.
Kodani A, Salomé Sirerol-Piquer M, Seol A, Garcia-Verdugo JM, Reiter JF
The EMBO journal 2013 Feb 20;32(4):597-607
The EMBO journal 2013 Feb 20;32(4):597-607
The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis.
Zhao H, Zhu L, Zhu Y, Cao J, Li S, Huang Q, Xu T, Huang X, Yan X, Zhu X
Nature cell biology 2013 Dec;15(12):1434-44
Nature cell biology 2013 Dec;15(12):1434-44
Misfolded Gβ is recruited to cytoplasmic dynein by Nudel for efficient clearance.
Wan Y, Yang Z, Guo J, Zhang Q, Zeng L, Song W, Xiao Y, Zhu X
Cell research 2012 Jul;22(7):1140-54
Cell research 2012 Jul;22(7):1140-54
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HL-60 cells were subjected to SDS PAGE followed by western blot with 12794-1-AP(CETN1 antibody) at dilution of 1:300
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using CETN1 antibody 12794-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line