12794-1-AP

antibody from Proteintech Group

Targeting: CETN1 CEN1, CETN

Provider product page for 12794-1-AP

Western blot

ELISA

Immunocytochemistry

Antibody data

Antibody Data
Antigen structure
References [52]
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Validations
Western blot [1]
Immunocytochemistry [1]

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Product number: 12794-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#12794-1-AP, RRID:AB_2077371
Product name: Centrin 1 antibody
Antibody type: Polyclonal
Description: Centrin 1 antibody (Cat. #12794-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IF, WB,ELISA.
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

CETN1 protein structure - 12794-1-AP shown in red.

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NuSAP Safeguards Centriole Integrity to Mediate CEP57-CEP152 Torus Recruitment for Proper Engagement.

Zhang S, Jiang Z, Yang Q, Zheng H, Wang M, Zhu C, Deng LW, Crasta KC, Liou YC
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Visualizing Cartwheel Disassembly Process During Mitosis in Fixed and Live Cells by Fluorescence Microscope.

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The Cilia-Associated Protein CCDC89 Is Dispensable for Male Fertility in Mice.

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Competitive antagonism of KAT7 crotonylation against acetylation affects procentriole formation and colorectal tumorigenesis.

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INF2 formin variants linked to human inherited kidney disease reprogram the transcriptome, causing mitotic chaos and cell death.

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Hornerin mediates phosphorylation of the polo-box domain in Plk1 by Chk1 to induce death in mitosis.

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Cell death and differentiation 2023 Sep;30(9):2151-2166

JNK regulates ciliogenesis through the interflagellar transport complex and actin networks.

Chatzifrangkeskou M, Kouis P, Skourides PA
The Journal of cell biology 2023 Nov 6;222(11)

K-Ras Binds Calmodulin-Related Centrin1 with Potential Implications for K-Ras Driven Cancer Cell Stemness.

Manoharan GB, Laurini C, Bottone S, Ben Fredj N, Abankwa DK
Cancers 2023 Jun 7;15(12)

Absence of CEP78 causes photoreceptor and sperm flagella impairments in mice and a human individual.

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CCDC15 localizes to the centriole inner scaffold and controls centriole length and integrity.

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The Journal of cell biology 2023 Dec 4;222(12)

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Scientific reports 2023 Aug 9;13(1):12884

CCHCR1-astrin interaction promotes centriole duplication through recruitment of CEP72.

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ENKD1 promotes CP110 removal through competing with CEP97 to initiate ciliogenesis.

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EMBO reports 2022 May 4;23(5):e54090

Molecular mechanisms underlying the role of the centriolar CEP164-TTBK2 complex in ciliopathies.

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Cartwheel disassembly regulated by CDK1-cyclin B kinase allows human centriole disengagement and licensing.

Huang F, Xu X, Xin G, Zhang B, Jiang Q, Zhang C
The Journal of biological chemistry 2022 Dec;298(12):102658

Loss of DRC1 function leads to multiple morphological abnormalities of the sperm flagella and male infertility in human and mouse.

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PLK4-phosphorylated NEDD1 facilitates cartwheel assembly and centriole biogenesis initiations.

Chi W, Wang G, Xin G, Jiang Q, Zhang C
The Journal of cell biology 2021 Jan 4;220(1)

O-GlcNAc transferase regulates centriole behavior and intraflagellar transport to promote ciliogenesis.

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ASK1-Mediated Phosphorylation Blocks HDAC6 Ubiquitination and Degradation to Drive the Disassembly of Photoreceptor Connecting Cilia.

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Homozygous mutations in DZIP1 can induce asthenoteratospermia with severe MMAF.

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Secreted Wnt6 mediates diabetes-associated centrosome amplification via its receptor FZD4.

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Centrosomal Localization of RXRα Promotes PLK1 Activation and Mitotic Progression and Constitutes a Tumor Vulnerability.

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Posterior Neocortex-Specific Regulation of Neuronal Migration by CEP85L Identifies Maternal Centriole-Dependent Activation of CDK5.

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Wdr47 Controls Neuronal Polarization through the Camsap Family Microtubule Minus-End-Binding Proteins.

Chen Y, Zheng J, Li X, Zhu L, Shao Z, Yan X, Zhu X
Cell reports 2020 Apr 21;31(3):107526

Bisphenol A disrupts mitotic progression via disturbing spindle attachment to kinetochore and centriole duplication in cancer cell lines.

Kim S, Gwon D, Kim JA, Choi H, Jang CY
Toxicology in vitro : an international journal published in association with BIBRA 2019 Sep;59:115-125

MICAL-L1 coordinates ciliogenesis by recruiting EHD1 to the primary cilium.

Xie S, Farmer T, Naslavsky N, Caplan S
Journal of cell science 2019 Nov 14;132(22)

Resting cells rely on the DNA helicase component MCM2 to build cilia.

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c-Cbl Acts as an E3 Ligase Against DDA3 for Spindle Dynamics and Centriole Duplication during Mitosis.

Gwon D, Hong J, Jang CY
Molecules and cells 2019 Dec 31;42(12):840-849

Parental centrioles are dispensable for deuterosome formation and function during basal body amplification.

Zhao H, Chen Q, Fang C, Huang Q, Zhou J, Yan X, Zhu X
EMBO reports 2019 Apr;20(4)

Vesicular trafficking plays a role in centriole disengagement and duplication.

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Cytoplasmic E2f4 forms organizing centres for initiation of centriole amplification during multiciliogenesis.

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Nature communications 2017 Jul 4;8:15857

DNA replication licensing factor Cdc6 and Plk4 kinase antagonistically regulate centrosome duplication via Sas-6.

Xu X, Huang S, Zhang B, Huang F, Chi W, Fu J, Wang G, Li S, Jiang Q, Zhang C
Nature communications 2017 Apr 27;8:15164

NudC regulates actin dynamics and ciliogenesis by stabilizing cofilin 1.

Zhang C, Zhang W, Lu Y, Yan X, Yan X, Zhu X, Liu W, Yang Y, Zhou T
Cell research 2016 Feb;26(2):239-53

The tumor suppressor proteins ASPP1 and ASPP2 interact with C-Nap1 and regulate centrosome linker reassembly.

Zhang Y, Wang Y, Wei Y, Ma J, Peng J, Wumaier R, Shen S, Zhang P, Yu L
Biochemical and biophysical research communications 2015 Mar 13;458(3):494-500

Centriolar satellites assemble centrosomal microcephaly proteins to recruit CDK2 and promote centriole duplication.

Kodani A, Yu TW, Johnson JR, Jayaraman D, Johnson TL, Al-Gazali L, Sztriha L, Partlow JN, Kim H, Krup AL, Dammermann A, Krogan NJ, Walsh CA, Reiter JF
eLife 2015 Aug 22;4

Kif3a interacts with Dynactin subunit p150 Glued to organize centriole subdistal appendages.

Kodani A, Salomé Sirerol-Piquer M, Seol A, Garcia-Verdugo JM, Reiter JF
The EMBO journal 2013 Feb 20;32(4):597-607

The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis.

Zhao H, Zhu L, Zhu Y, Cao J, Li S, Huang Q, Xu T, Huang X, Yan X, Zhu X
Nature cell biology 2013 Dec;15(12):1434-44

Misfolded Gβ is recruited to cytoplasmic dynein by Nudel for efficient clearance.

Wan Y, Yang Z, Guo J, Zhang Q, Zeng L, Song W, Xiao Y, Zhu X
Cell research 2012 Jul;22(7):1140-54

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HL-60 cells were subjected to SDS PAGE followed by western blot with 12794-1-AP(CETN1 antibody) at dilution of 1:300
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of Hela cells, using CETN1 antibody 12794-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line