GTX11257

antibody from GeneTex

Targeting: CETN1 CEN1, CETN

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX11257

Provider

GeneTex

Proper citation

GeneTex Cat#GTX11257, RRID:AB_368851

Product name

Centrin 1 antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Lysate of HEK-293T cells overexpressing human centrin-1 was separated on SDS-PAGE, blotted with Anti-Centrin (GTX11257) and developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. Antibody 0.3 μg/mL 2. Antibody 0.3 μg/mL + centrin1 immunizing peptide (human, 157-172)

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Lysates of HT-29 (lanes 1,2), HEK-293T (lanes (3-4) and HeLa (lanes 5-6) cells were separated on SDS-PAGE, blotted with 0.3 μg/mL Anti-Centrin (GTX11257) and developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate. Lanes 1,3,5. Antibody 0.3 μg/mL 2,4,6. Antibody 0.3 μg/mL + centrin1 immunizing peptide (human, 157-172)

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

NIH3T3 cells were fixed and stained with 6 μg/mL Anti-Centrin (GTX11257). The antibody was developed using Goat Anti-Rabbit IgG, Atto488 conjμgate. Nuclear staining with DAPI.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HeLa cells were fixed and stained with 6 μg/mL Anti-Centrin (GTX11257). The antibody was developed using Goat Anti-Rabbit IgG, Atto488 conjμgate. Nuclear staining with DAPI.