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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- GTX114316 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX114316, RRID:AB_10619101
- Product name
- Centrin 1 antibody [N1C3]
- Antibody type
- Polyclonal
- Reactivity
- Human, Bovine
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: JurKat 12% SDS PAGE Centrin-1 antibody GTX114316 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Centrin 1 antibody detects Centrin 1 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 15 % SDS-PAGE, and blotted with Centrin 1 antibody (GTX114316) diluted by 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Centrin 1 antibody detects Centrin 1 protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 15% SDS-PAGE, and the membrane was blotted with Centrin 1 antibody (GTX114316) diluted at a dilution of 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Centrin 1 antibody [N1C3] detects Centrin 1 protein at centrosome by immunofluorescent analysis.Sample: A431 cells were fixed in ice-cold MeOH for 5 min.Green: Centrin 1 stained by Centrin 1 antibody [N1C3] (GTX114316) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of Centrin-1 protein from 293T whole cell extracts using 5 £gg of Centrin-1 antibody [N1C3] (GTX114316).Western blot analysis was performed using Centrin-1 antibody [N1C3] (GTX114316).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
