Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [6]
Submit
Validation data
Reference
Comment
Report error
- Product number
- LS-C742072 - Provider product page
- Provider
- LSBio
- Product name
- BTG3 Antibody (clone 10A8) LS-C742072
- Antibody type
- Monoclonal
- Description
- Protein A affinity chromatography
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 10A8
- Storage
- Long term: store at -20°C for up to 1 years. Reconstituted: store at 2°C to 8°C for up to 3 weeks or at -20°C for up to 1 year. Avoid freeze/thaw cycles.
No comments: Submit comment
Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Independent validation of Human BTG3 Antibody (10A8) and Human BTG3 Antibody (12B4) with Human BTG3 recombinant protein. Lane 1: 50 ng Human BTG3 recombinant protein Lane 2: 25 ng Human BTG3 recombinant protein Lane 3: 10 ng Human BTG3 recombinant protein Lane 4: 50 ng Human BTG3 recombinant protein Lane 5: 25 ng Human BTG3 recombinant protein Lane 6: 10 ng Human BTG3 recombinant protein Primary Antibody: Lane 1~3: Human BTG3 Antibody (12B4) 1 µg/ml Lane 4~6: Human BTG3 Antibody (10A8) (H&L) [IRDye8°°] (Licor,926-32211)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Independent validation of Human BTG3 Antibody (10A8) and Human BTG3 Antibody (12B4) with Human BTG3 recombinant protein. Lane 1: 50 ng Human BTG3 recombinant protein Lane 2: 25 ng Human BTG3 recombinant protein Lane 3: 10 ng Human BTG3 recombinant protein Lane 4: 50 ng Human BTG3 recombinant protein Lane 5: 25 ng Human BTG3 recombinant protein Lane 6: 10 ng Human BTG3 recombinant protein Primary Antibody: Lane 1~3: Human BTG3 Antibody (12B4) 1 µg/ml Lane 4~6: Human BTG3 Antibody (10A8) (H&L) [IRDye8°°] (Licor,926-32211)
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Standard curve of BTG3 Sandwich ELISA. The BTG3 Sandwich ELISA assay is developed by using Human BTG3 Antibody (12B4) and Biotin conjugated Human BTG3 Antibody (10A8) as capture and detect antibody, respectively. From the experimental results, Human BTG3 Antibody (12B4) and Human BTG3 Antibody (10A8) identify different epitopes. The sensitivity is 1-3 ng/ml and the detection range is 0-100 ng/ml.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- ELISA binding of Human BTG3 Antibody (10A8) with Human BTG3 recombinant protein. Coating antigen: BTG3, 1 µg/ml. BTG3 antibody dilution start from 1000 ng/ml, EC50= 30.87 ng/ml.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Standard curve of BTG3 Sandwich ELISA. The BTG3 Sandwich ELISA assay is developed by using Human BTG3 Antibody (12B4) and Biotin conjugated Human BTG3 Antibody (10A8) as capture and detect antibody, respectively. From the experimental results, Human BTG3 Antibody (12B4) and Human BTG3 Antibody (10A8) identify different epitopes. The sensitivity is 1-3 ng/ml and the detection range is 0-100 ng/ml.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- ELISA binding of Human BTG3 Antibody (10A8) with Human BTG3 recombinant protein. Coating antigen: BTG3, 1 µg/ml. BTG3 antibody dilution start from 1000 ng/ml, EC50= 30.87 ng/ml.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Standard curve of BTG3 Sandwich ELISA. The BTG3 Sandwich ELISA assay is developed by using Human BTG3 Antibody (12B4) and Biotin conjugated Human BTG3 Antibody (10A8) as capture and detect antibody, respectively. From the experimental results, Human BTG3 Antibody (12B4) and Human BTG3 Antibody (10A8) identify different epitopes. The sensitivity is 1-3 ng/ml and the detection range is 0-100 ng/ml.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- ELISA binding of Human BTG3 Antibody (10A8) with Human BTG3 recombinant protein. Coating antigen: BTG3, 1 µg/ml. BTG3 antibody dilution start from 1000 ng/ml, EC50= 30.87 ng/ml.
