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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [4]
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- Product number
- PA5-109749 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- G6PC3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Immunogen sequence: TLGAGIVIAE ALQNQLAWLE NVWLWITFLG DPKILFLFYF PAAYYASRRV GIAVLWISLI TEWLNLIFKW FLFGDRPFWW VHESGYYSQA PAQVHQFPSS
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.62 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of G6PC3 in extracts of 293T cells. Samples were incubated with G6PC3 Polyclonal antibody (Product # PA5-109749) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of G6PC3 in extracts of various cell lines. Samples were incubated with G6PC3 Polyclonal antibody (Product # PA5-109749) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of G6PC3 in U2OS cells. Samples were incubated with G6PC3 Polyclonal antibody (Product # PA5-109749) using a dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of G6PC3 in C6 cells. Samples were incubated with G6PC3 Polyclonal antibody (Product # PA5-109749) using a dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of G6PC3 in NIH/3T3 cells. Samples were incubated with G6PC3 Polyclonal antibody (Product # PA5-109749) using a dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of G6PC3 in A-549 cells. Samples were incubated with G6PC3 Polyclonal antibody (Product # PA5-109749) using a dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
