GTX109641

antibody from GeneTex

Targeting: TUBB8 bA631M21.2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX109641

Provider

GeneTex

Proper citation

GeneTex Cat#GTX109641, RRID:AB_1951741

Product name

beta Tubulin 8 antibody [C2C3], C-term

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

beta Tubulin 8 antibody [C2C3], C-term detects RP11-631M21.2 protein by Western blot analysis.A. 30 ?g Neuro2A whole cell lysate/extract B. 30 ?g GL261 whole cell lysate/extractC. 30 ?g C8D30 whole cell lysate/extractD. 30 ?g NIH-3T3 whole cell lysate/extractE. 30 ?g BCL-1 whole cell lysate/extractF. 30 ?g Raw264.7 whole cell lysate/extractG. 30 ?g C2C12 whole cell lysate/extract10 % SDS-PAGEbeta Tubulin 8 antibody [C2C3], C-term (GTX109641) dilution: 1:1000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample(30 ug whole cell lysate)A:A431(GTX27909)B:H129910% SDS PAGEGTX109641 diluted at 1:1000

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using beta Tubulin 8(GTX109641) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

beta Tubulin 8 antibody [C2C3], C-term detects beta Tubulin 8 protein at cytoskeleton by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: beta Tubulin 8 protein stained by beta Tubulin 8 antibody [C2C3], C-term (GTX109641) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.

Immunoprecipitation

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

beta Tubulin 8 antibody immunoprecipitates beta Tubulin 8 protein in IP experiments.IP Sample: 293T whole cell lysate/extract A. 40 £gg 293T whole cell lysate/extractB. Control with 2 £gg of preimmune rabbit IgGC. Immunoprecipitation of beta Tubulin 8 protein by 2 £gg of beta Tubulin 8 antibody (GTX109641)10% SDS-PAGE The immunoprecipitated beta Tubulin 8 protein was detected by beta Tubulin 8 antibody (GTX109641) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

beta Tubulin 8 antibody [C2C3], C-term detects RP11-631M21.2 protein at cytosol on Mahlarvu xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Mahlarvu xenograft. beta Tubulin 8 antibody [C2C3], C-term (GTX109641) dilution: 1:500.