Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA319489 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal anti-DELTA-4 antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal anti-DELTA-4 antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- DLL4
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- NULL
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot of Rabbit anti-Delta-4 antibody. Lane 1: mouse pancreatic tissue. Load: 20 ug per lane. Primary antibody: Delta-4 antibody at 1:500 for overnight at 4°C. Secondary antibody: HRP-labeled Goat anti-Rabbit IgG secondary antibody at 1:5,000 for 1 hour at RT. A chemiluminescence system was used for signal detection using a 3 min exposure time. Block: 5% Goat Serum overnight at 4°C. Predicted/Observed size: 74 kDa, 70 kDa for Delta 4. Other band(s): none.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Affinity Purified anti-Delta-4 antibody was used at 20 ug/ml to detect Delta-4 in a variety of tissues including colon, liver, skeletal muscle, ovary, pancreas, prostate, testes, thymus, tonsil and uterus. In contrast to reported findings, no staining was observed in vascular tissue. This image shows Delta-4 staining of human ovary. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.
- Validation comment
- IHC
