Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-97664 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DLL4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.3 mg/mL
- Storage
- -20°C or -80°C if preferred
Submitted references Notch1 signaling determines the plasticity and function of fibroblasts in diabetic wounds.
Shao H, Li Y, Pastar I, Xiao M, Prokupets R, Liu S, Yu K, Vazquez-Padron RI, Tomic-Canic M, Velazquez OC, Liu ZJ
Life science alliance 2020 Dec;3(12)
Life science alliance 2020 Dec;3(12)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of DLL4 using a DLL4 Polyclonal antibody (Product # PA5-97664) at a concentration of 4 µg/mL. Positive WB detected in: HEK293 whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 75 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of DLL4 in MCF-7 cells using a DLL4 polyclonal antibody (Product # PA5-97664) at a dilution of 1:100. Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L) secondary antibody was used.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1. Differential Notch pathway activities in fibroblasts of chronic diabetic skin wounds versus non-diabetic skin and wounds. (A) High Notch pathway activity in diabetic foot ulcer fibroblasts (DFUF) versus low Notch pathway activity in normal foot fibroblasts (NFF). Expression of Notch pathway components in three DFUF and three NFF were assessed by immunoblot. beta-actin was used as a loading control. The band of each molecule is shown. (B) Inhibition of the Notch pathway activity, reflected by decreased levels of N1 IC and Hey-1, in DFUF by DAPT and Jag 1 neutralizing Ab. Compared with DAPT, Jag 1 neutralizing Ab only achieved a partial inhibition. (C) Representative immunostaining images show that fibroblasts (green) express higher levels of Hes-1 (red) in skin at the edge of diabetic foot ulcer tissue than that in non-diabetic foot skin. Highlighted areas show fibroblasts in reticular layers. (D) Representative immunostaining images show that fibroblasts (green) express higher levels of Hes-1 (red) in wounds of diabetic mice (db/db and NOD) but not in non-diabetic acute wound and ischemic chronic wounds in C57 BL6 mice. Wound tissues were harvested at day 7. Highlighted areas show fibroblasts in granulation tissues.