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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00003306-M06 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00003306-M06, RRID:AB_464427
- Product name
- HSPA2 monoclonal antibody (M06), clone S51
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant HSPA2.
- Antigen sequence
MSARGPAIGIDLGTTYSCVGVFQHGKVEIIANDQG
NRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTIFD
AKRLIGRKFGDATVQSDMKHWPFRVVSEGGKPKVQ
VEYKGETKTFFPEEISSMVLTKMKEIAEAYLGGKV
HSAVITVPAYFNDSQRQATKDAGTITGLNVLRIIN
EPTAAAIAYGLDKKGCAGGEKNVLIFDLGGGTFDV
SILTIEDGIFEVKSTAGDTHLGGEDFDNRMVSHLA
EEFKRKHKKDIGPNKRAVRRLRTACERAKRTLSSS
TQASIEIDSLYEGVDFYTSITRARFEELNADLFRG
TLEPVEKALRDAKLDKGQIQEIVLVGGSTRIPKIQ
KLLQDFFNGKELNKSINPDEAVAYGAAVQAAILIG
DKSENVQDLLLLDVTPLSLGIETAGGVMTPLIKRN
TTIPTKQTQTFTTYSDNQSSVLVQVYEGERAMTKD
NNLLGKFDLTGIPPAPRGVPQIEVTFDIDANGILN
VTAADKSTGKENKITITNDKGRLSKDDIDRMVQEA
ERYKSEDEANRDRVAAKNALESYTYNIKQTVEDEK
LRGKISEQDKNKILDKCQEVINWLDRNQMAEKDEY
EHKQKELERVCNPIISKLYQGGPGGGSGGGGSGAS
GGPTIEEVD- Isotype
- IgG
- Antibody clone number
- S51
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Bat3 deficiency accelerates the degradation of Hsp70-2/HspA2 during spermatogenesis.
Sasaki T, Marcon E, McQuire T, Arai Y, Moens PB, Okada H
The Journal of cell biology 2008 Aug 11;182(3):449-58
The Journal of cell biology 2008 Aug 11;182(3):449-58
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- HSPA2 monoclonal antibody (M06), clone S51. Western Blot analysis of HSPA2 expression in different cell lines.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- HSPA2 monoclonal antibody (M06), clone S51. Western Blot analysis of HSPA2 expression in HeLa(Cat # L013V1 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged HSPA2 is approximately 0.3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of HSPA2 transfected lysate using anti-HSPA2 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with HSPA2 MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to HSPA2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
