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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00003306-M03 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00003306-M03, RRID:AB_464330
- Product name
- HSPA2 monoclonal antibody (M03), clone 2D11
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant HSPA2.
- Antigen sequence
MSARGPAIGIDLGTTYSCVGVFQHGKVEIIANDQG
NRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTIFD
AKRLIGRKFGDATVQSDMKHWPFRVVSEGGKPKVQ
VEYKGETKTFFPEEISSMVLTKMKEIAEAYLGGKV
HSAVITVPAYFNDSQRQATKDAGTITGLNVLRIIN
EPTAAAIAYGLDKKGCAGGEKNVLIFDLGGGTFDV
SILTIEDGIFEVKSTAGDTHLGGEDFDNRMVSHLA
EEFKRKHKKDIGPNKRAVRRLRTACERAKRTLSSS
TQASIEIDSLYEGVDFYTSITRARFEELNADLFRG
TLEPVEKALRDAKLDKGQIQEIVLVGGSTRIPKIQ
KLLQDFFNGKELNKSINPDEAVAYGAAVQAAILIG
DKSENVQDLLLLDVTPLSLGIETAGGVMTPLIKRN
TTIPTKQTQTFTTYSDNQSSVLVQVYEGERAMTKD
NNLLGKFDLTGIPPAPRGVPQIEVTFDIDANGILN
VTAADKSTGKENKITITNDKGRLSKDDIDRMVQEA
ERYKSEDEANRDRVAAKNALESYTYNIKQTVEDEK
LRGKISEQDKNKILDKCQEVINWLDRNQMAEKDEY
EHKQKELERVCNPIISKLYQGGPGGGSGGGGSGAS
GGPTIEEVD- Isotype
- IgG
- Antibody clone number
- 2D11
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Polychlorinated biphenyls have inhibitory effect on testicular steroidogenesis by downregulation of P450(17alpha) and P450(scc).
Han DY, Kang SR, Park OS, Cho JH, Won CK, Park HS, Park KI, Kim EH, Kim GS
Toxicology and industrial health 2010 Jun;26(5):287-96
Toxicology and industrial health 2010 Jun;26(5):287-96
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- HSPA2 monoclonal antibody (M03), clone 2D11 Western Blot analysis of HSPA2 expression in HepG2 ( Cat # L019V1 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged HSPA2 is approximately 3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of HSPA2 transfected lysate using anti-HSPA2 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with HSPA2 MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
