Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [5]
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Validation data
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- Product number
- NBP1-86185 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-86185, RRID:AB_11019782
- Product name
- Rabbit Polyclonal HSPA2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human, mouse, rat HSPA2 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Hypoxia drives the assembly of the multienzyme purinosome complex.
Expression, function, and regulation of the testis-enriched heat shock HSPA2 gene in rodents and humans.
HSP70-binding protein HSPBP1 regulates chaperone expression at a posttranslational level and is essential for spermatogenesis.
The molecular chaperone Hsp90α is required for meiotic progression of spermatocytes beyond pachytene in the mouse.
Systematically generated antibodies against human gene products: high throughput screening on sections from the rat nervous system.
Doigneaux C, Pedley AM, Mistry IN, Papayova M, Benkovic SJ, Tavassoli A
The Journal of biological chemistry 2020 Jul 10;295(28):9551-9566
The Journal of biological chemistry 2020 Jul 10;295(28):9551-9566
Expression, function, and regulation of the testis-enriched heat shock HSPA2 gene in rodents and humans.
Scieglinska D, Krawczyk Z
Cell stress & chaperones 2015 Mar;20(2):221-35
Cell stress & chaperones 2015 Mar;20(2):221-35
HSP70-binding protein HSPBP1 regulates chaperone expression at a posttranslational level and is essential for spermatogenesis.
Rogon C, Ulbricht A, Hesse M, Alberti S, Vijayaraj P, Best D, Adams IR, Magin TM, Fleischmann BK, Höhfeld J
Molecular biology of the cell 2014 Aug 1;25(15):2260-71
Molecular biology of the cell 2014 Aug 1;25(15):2260-71
The molecular chaperone Hsp90α is required for meiotic progression of spermatocytes beyond pachytene in the mouse.
Grad I, Cederroth CR, Walicki J, Grey C, Barluenga S, Winssinger N, De Massy B, Nef S, Picard D
PloS one 2010 Dec 31;5(12):e15770
PloS one 2010 Dec 31;5(12):e15770
Systematically generated antibodies against human gene products: high throughput screening on sections from the rat nervous system.
Mulder J, Wernérus H, Shi TJ, Pontén F, Hober S, Uhlén M, Hökfelt T
Neuroscience 2007 Jun 8;146(4):1689-703
Neuroscience 2007 Jun 8;146(4):1689-703
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HSPA2 Antibody [NBP1-86185] - Analysis in human testis tissue.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HSPA2 Antibody [NBP1-86185] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HSPA2 Antibody [NBP1-86185] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HSPA2 Antibody [NBP1-86185] - Analysis in human testis and pancreas tissues. Corresponding HSPA2 RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HSPA2 Antibody [NBP1-86185] - Staining of human cerebral cortex shows moderate nuclear positivity in glial cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HSPA2 Antibody [NBP1-86185] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HSPA2 Antibody [NBP1-86185] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: HSPA2 Antibody [NBP1-86185] - Staining of mouse brain shows moderate to strong positivity in more than 75 % of neurons in the thalamus.