14-9054-82

MSR1 antibody from Invitrogen Antibodies
CD204, SCARA1
Immunohistochemistry
Recommended by provider

Antibody data

Product number
14-9054-82
Provider
Invitrogen Antibodies
Product name
Anti-CD204 Monoclonal Antibody (J5HTR3), eBioscience™
Provider product page
Invitrogen Antibodies - 14-9054-82
Antibody type
Monoclonal
Antigen
Other
Description
This monoclonal antibody J5HTR3 reacts with human CD204 also known as Macrophage Scavenger Receptor 1 (MSR1), Scavenger Receptor Type A (SR-A), or SCARA1. It is expressed by multiple populations of macrophages and dendritic cells. CD204 belongs to the class A family of scavenger receptors, a group of five homotrimetric transmembrane proteins that contain a short cytoplasmic tail and extracellular C -terminus. Other members of this family include: MARCO (SCARA2), CSR (SCARA3), SRCL (SCARA4) and SCARA5. CD204 binds and internalizes oxidized low density lipoproteins (LDL), which drives macrophage differentiation into foam cells, secretion of inflammatory cytokines, and development of atherosclerotic lesions. In addition to oxidized LDL, CD204 binds and uptakes a wide variety of macromolecules, including bacterial cell wall components, viral DNA, calciprotein complexes, apoptotic cells, and beta-amyloid fibrils. This broad binding specificity implicates CD204-expressing macrophages in a number of physiological processes and pathologies, such as host defense, inflammation, bone remodeling, atherosclerosis, sepsis, and Alzheimer's disease. In humans, CD204 exists as 3 splice variants. The more ubiquitous full length isoform (type I, SR-AI), a shorter isoform (type II, SR-AII) that lacks the C-terminal cysteine-rich domain, and the shortest isoform (type III, SR-AIII). Isoforms I and II bind modified LDL with similar affinity, whereas isoform III is not expressed on the cell surface. This monoclonal antibody J5HTR3 binds both type I and type II isoforms (isoform III has not been tested). This J5HTR3 antibody has been tested by immunohistochemical staining of formalin-fixed paraffin embedded human tissue using high pH antigen retrieval and can be used at less than or equal to 0.5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
J5HTR3
Vial size
100 µg
Concentration
0.5 mg/ml
Storage
4° C, do not freeze
Provider Type Product Number
- No reagents -