14795-1-AP

antibody from Proteintech Group

Targeting: MRPL12 MRPL7, MRPL7/L12, RPML12

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

14795-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#14795-1-AP, RRID:AB_2250805

Product name

MRPL12 antibody

Antibody type

Polyclonal

Description

KD/KO validated MRPL12 antibody (Cat. #14795-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Suppressive Genetic Interactions Between Haploinsufficient Mitochondrial Genes Encoded in the 22q11.2 Microdeletion Locus Define Brain and Cardiac Phenotypes.

Wynne M, Zlatic SA, Park AS, Crocker A, Mendez-Vazquez H, Liporace E, Gokhale A, Tower-Gilchrist C, Robinette M, Purcell RH, Bassell GJ, Werner E, Kwong JQ, Faundez V
bioRxiv : the preprint server for biology 2026 Jan 9;

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Mitochondrial ribosomal protein L12 mediates metabolic reorganization in clear cell renal cell carcinoma by regulating mitochondrial biosynthesis.

Sun S, Su D, Dong T, Wang B, Ji X, Chu L, Lu S, Zhang T, Sun X, Liu Y, Wan Q
Cell communication and signaling : CCS 2025 Oct 14;23(1):435

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High MRPL12 expression drives esophageal cancer proliferation, invasion and migration.

Wang G, Sun X, Liu Q, Pang Z, Huang Y, Lv L, Zhang H, Liu H, Zhu L
Bulletin du cancer 2025 Nov;112(11):1240-1250

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Microprotein SMIM26 drives oxidative metabolism via serine-responsive mitochondrial translation.

Nah J, Mahendran S, Kerouanton B, Cui L, Hock DH, Cabrera-Orefice A, Dunlap K, Robinson D, Tung DWH, Leong SH, Tan KY, Chothani SP, Sun J, Dziegowska A, Leszczynska G, Rackham OJL, Wittig I, Dedon P, Ducker GS, Stroud DA, Ho L
Molecular cell 2025 Jul 17;85(14):2759-2775.e12

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Super-resolution microscopy of mitochondrial mRNAs.

Stoldt S, Maass F, Weber M, Dennerlein S, Ilgen P, Gärtner J, Canfes A, Schweighofer SV, Jans DC, Rehling P, Jakobs S
Nature communications 2025 Jul 10;16(1):6391

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MRPL12 K163 acetylation inhibits ccRCC via driving mitochondrial metabolic reprogramming.

Ji X, Xue F, Wang Y, Gao D, Sun J, Dong T, Mu Q, Xu Q, Sun S, Liu Y, Wan Q
Cell death & disease 2025 Aug 26;16(1):646

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Role of mitochondrial ribosomal protein L7/L12 (MRPL12) in diabetic ischemic heart disease.

Rai AK, Sanghvi S, Muthukumaran NS, Chandrasekera D, Kadam A, Kishore J, Kyriazis ID, Tomar D, Ponnalagu D, Shettigar V, Khan M, Singh H, Goukassian D, Katare R, Garikipati VNS
Free radical biology & medicine 2024 Sep;222:531-538

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UBASH3B-mediated MRPL12 Y60 dephosphorylation inhibits LUAD development by driving mitochondrial metabolism reprogramming.

Ji X, Zhang T, Sun J, Song X, Ma G, Xu L, Cao X, Jing Y, Xue F, Zhang W, Sun S, Wan Q, Liu Y
Journal of experimental & clinical cancer research : CR 2024 Sep 30;43(1):268

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Poly-GP accumulation due to C9orf72 loss of function induces motor neuron apoptosis through autophagy and mitophagy defects.

de Calbiac H, Renault S, Haouy G, Jung V, Roger K, Zhou Q, Campanari ML, Chentout L, Demy DL, Marian A, Goudin N, Edbauer D, Guerrera C, Ciura S, Kabashi E
Autophagy 2024 Oct;20(10):2164-2185

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The role of lysine acetylation in the function of mitochondrial ribosomal protein L12.

Paluch KV, Platz KR, Rudisel EJ, Erdmann RR, Laurin TR, Dittenhafer-Reed KE
Proteins 2024 May;92(5):583-592

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Mitochondrial ribosomal protein L12 potentiates hepatocellular carcinoma by regulating mitochondrial biogenesis and metabolic reprogramming.

Ji X, Yang Z, Li C, Zhu S, Zhang Y, Xue F, Sun S, Fu T, Ding C, Liu Y, Wan Q
Metabolism: clinical and experimental 2024 Mar;152:155761

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Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria.

Poerschke S, Oeljeklaus S, Cruz-Zaragoza LD, Schenzielorz A, Dahal D, Hillen HS, Das H, Kremer LS, Valpadashi A, Breuer M, Sattmann J, Richter-Dennerlein R, Warscheid B, Dennerlein S, Rehling P
Molecular cell 2024 Jan 18;84(2):345-358.e5

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A roadmap for ribosome assembly in human mitochondria.

Lavdovskaia E, Hanitsch E, Linden A, Pašen M, Challa V, Horokhovskyi Y, Roetschke HP, Nadler F, Welp L, Steube E, Heinrichs M, Mai MM, Urlaub H, Liepe J, Richter-Dennerlein R
Nature structural & molecular biology 2024 Dec;31(12):1898-1908

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A kinetic dichotomy between mitochondrial and nuclear gene expression processes.

McShane E, Couvillion M, Ietswaart R, Prakash G, Smalec BM, Soto I, Baxter-Koenigs AR, Choquet K, Churchman LS
Molecular cell 2024 Apr 18;84(8):1541-1555.e11

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Human Mitoribosome Profiling: A Re-engineered Approach Tailored to Study Mitochondrial Translation.

Soto I, Couvillion M, Stirling Churchman L
Methods in molecular biology (Clifton, N.J.) 2023;2661:257-280

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A kinetic dichotomy between mitochondrial and nuclear gene expression drives OXPHOS biogenesis.

McShane E, Couvillion M, Ietswaart R, Prakash G, Smalec BM, Soto I, Baxter-Koenigs AR, Choquet K, Churchman LS
bioRxiv : the preprint server for biology 2023 Sep 6;

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C-IGF1R encoded by cIGF1R acts as a molecular switch to restrict mitophagy of drug-tolerant persister tumour cells in non-small cell lung cancer.

Wang H, Liang Y, Zhang T, Yu X, Song X, Chen Y, Mao Q, Xia W, Chen B, Xu L, Dong G, Jiang F
Cell death and differentiation 2023 Nov;30(11):2365-2381

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Regulators of mitonuclear balance link mitochondrial metabolism to mtDNA expression.

Kramer NJ, Prakash G, Isaac RS, Choquet K, Soto I, Petrova B, Merens HE, Kanarek N, Churchman LS
Nature cell biology 2023 Nov;25(11):1575-1589

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BOLA3 and NFU1 link mitoribosome iron-sulfur cluster assembly to multiple mitochondrial dysfunctions syndrome.

Zhong H, Janer A, Khalimonchuk O, Antonicka H, Shoubridge EA, Barrientos A
Nucleic acids research 2023 Nov 27;51(21):11797-11812

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GTPBP8 is required for mitoribosomal biogenesis and mitochondrial translation.

Wang L, Hilander T, Liu X, Tsang HY, Eriksson O, Jackson CB, Varjosalo M, Zhao H
Cellular and molecular life sciences : CMLS 2023 Nov 16;80(12):361

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MRPL12-ANT3 interaction involves in acute kidney injury via regulating MPTP of tubular epithelial cells.

Ji X, Chu L, Su D, Sun J, Song P, Sun S, Wang Y, Mu Q, Liu Y, Wan Q
iScience 2023 May 19;26(5):106656

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P53 regulates mitochondrial biogenesis via transcriptionally induction of mitochondrial ribosomal protein L12.

Han Y, Liu Y, Zhen J, Hou S, Zhang B, Cui Z, Wan Q, Feng H
Experimental cell research 2022 Sep 1;418(1):113249

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Balanced mitochondrial and cytosolic translatomes underlie the biogenesis of human respiratory complexes.

Soto I, Couvillion M, Hansen KG, McShane E, Moran JC, Barrientos A, Churchman LS
Genome biology 2022 Aug 9;23(1):170

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ING2 Controls Mitochondrial Respiration via Modulating MRPL12 Ubiquitination in Renal Tubular Epithelial Cells.

Yang Y, Li C, Gu X, Zhen J, Zhu S, Lv T, Wan Q, Liu Y
Frontiers in cell and developmental biology 2021;9:700195

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Linear Density Sucrose Gradients to Study Mitoribosomal Biogenesis in Tissue-Specific Knockout Mice.

Ruzzenente B, Metodiev MD
Methods in molecular biology (Clifton, N.J.) 2021;2224:47-60

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An in vitro system to silence mitochondrial gene expression.

Cruz-Zaragoza LD, Dennerlein S, Linden A, Yousefi R, Lavdovskaia E, Aich A, Falk RR, Gomkale R, Schöndorf T, Bohnsack MT, Richter-Dennerlein R, Urlaub H, Rehling P
Cell 2021 Nov 11;184(23):5824-5837.e15

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Actin-granule formation is an additional step in cardiac myofibroblast differentiation.

He L, Liu R, Yue H, Ren S, Zhu G, Guo Y, Qin C
Annals of translational medicine 2021 Jan;9(2):165

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Transcription of MRPL12 regulated by Nrf2 contributes to the mitochondrial dysfunction in diabetic kidney disease.

Gu X, Liu Y, Wang N, Zhen J, Zhang B, Hou S, Cui Z, Wan Q, Feng H
Free radical biology & medicine 2021 Feb 20;164:329-340

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RCC1L (WBSCR16) isoforms coordinate mitochondrial ribosome assembly through their interaction with GTPases.

Reyes A, Favia P, Vidoni S, Petruzzella V, Zeviani M
PLoS genetics 2020 Jul;16(7):e1008923

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SQSTM1/p62 Controls mtDNA Expression and Participates in Mitochondrial Energetic Adaption via MRPL12.

Ma Y, Zhu S, Lv T, Gu X, Feng H, Zhen J, Xin W, Wan Q
iScience 2020 Aug 21;23(8):101428

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RNase H1 Regulates Mitochondrial Transcription and Translation via the Degradation of 7S RNA.

Reyes A, Rusecka J, Tońska K, Zeviani M
Frontiers in genetics 2019;10:1393

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METTL15 introduces N4-methylcytidine into human mitochondrial 12S rRNA and is required for mitoribosome biogenesis.

Van Haute L, Hendrick AG, D'Souza AR, Powell CA, Rebelo-Guiomar P, Harbour ME, Ding S, Fearnley IM, Andrews B, Minczuk M
Nucleic acids research 2019 Nov 4;47(19):10267-10281

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Stress signaling and cellular proliferation reverse the effects of mitochondrial mistranslation.

Ferreira N, Perks KL, Rossetti G, Rudler DL, Hughes LA, Ermer JA, Scott LH, Kuznetsova I, Richman TR, Narayana VK, Abudulai LN, Shearwood AJ, Cserne Szappanos H, Tull D, Yeoh GC, Hool LC, Filipovska A, Rackham O
The EMBO journal 2019 Dec 16;38(24):e102155

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Ammonium accumulation and chemokine decrease in culture media of Gcdh(-/-) 3D reaggregated brain cell cultures.

Cudré-Cung HP, Remacle N, do Vale-Pereira S, Gonzalez M, Henry H, Ivanisevic J, Schmiesing J, Mühlhausen C, Braissant O, Ballhausen D
Molecular genetics and metabolism 2019 Apr;126(4):416-428

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The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.

Lavdovskaia E, Kolander E, Steube E, Mai MM, Urlaub H, Richter-Dennerlein R
Nucleic acids research 2018 Sep 19;46(16):8471-8482

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Concerted regulation of mitochondrial and nuclear non-coding RNAs by a dual-targeted RNase Z.

Siira SJ, Rossetti G, Richman TR, Perks K, Ermer JA, Kuznetsova I, Hughes L, Shearwood AJ, Viola HM, Hool LC, Rackham O, Filipovska A
EMBO reports 2018 Oct;19(10)

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Mitochondrial tyrosyl-DNA phosphodiesterase 2 and its TDP2(S) short isoform.

Huang SN, Dalla Rosa I, Michaels SA, Tulumello DV, Agama K, Khiati S, Jean SR, Baechler SA, Factor VM, Varma S, Murai J, Miller Jenkins LM, Kelley SO, Pommier Y
EMBO reports 2018 Mar;19(3)

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Ammonium accumulation is a primary effect of 2-methylcitrate exposure in an in vitro model for brain damage in methylmalonic aciduria.

Cudré-Cung HP, Zavadakova P, do Vale-Pereira S, Remacle N, Henry H, Ivanisevic J, Tavel D, Braissant O, Ballhausen D
Molecular genetics and metabolism 2016 Sep;119(1-2):57-67

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Mitochondrial Ribosomal Protein L12 Is Required for POLRMT Stability and Exists as Two Forms Generated by Alternative Proteolysis during Import.

Nouws J, Goswami AV, Bestwick M, McCann BJ, Surovtseva YV, Shadel GS
The Journal of biological chemistry 2016 Jan 8;291(2):989-97

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Hierarchical RNA Processing Is Required for Mitochondrial Ribosome Assembly.

Rackham O, Busch JD, Matic S, Siira SJ, Kuznetsova I, Atanassov I, Ermer JA, Shearwood AM, Richman TR, Stewart JB, Mourier A, Milenkovic D, Larsson NG, Filipovska A
Cell reports 2016 Aug 16;16(7):1874-90

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MPV17L2 is required for ribosome assembly in mitochondria.

Dalla Rosa I, Durigon R, Pearce SF, Rorbach J, Hirst EM, Vidoni S, Reyes A, Brea-Calvo G, Minczuk M, Woellhaf MW, Herrmann JM, Huynen MA, Holt IJ, Spinazzola A
Nucleic acids research 2014 Jul;42(13):8500-15

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Transcription-independent role for human mitochondrial RNA polymerase in mitochondrial ribosome biogenesis.

Surovtseva YV, Shadel GS
Nucleic acids research 2013 Feb 1;41(4):2479-88

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 14795-1-AP(MRPL12 antibody) at dilution of 1:500

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using MRPL12 antibody 14795-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human colon cancer using 14795-1-AP(MRPL12 antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human colon cancer using 14795-1-AP(MRPL12 antibody) at dilution of 1:100 (under 40x lens)

Sample type

tissue