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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00006182-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00006182-M01, RRID:AB_425660
- Product name
- MRPL12 monoclonal antibody (M01), clone 3B12-1A3
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant MRPL12.
- Antigen sequence
MLPAAARPLWGPCLGLRAAAFRLARRQVPCVCAVR
HMRSSGHQRCEALAGAPLDNAPKEYPPKIQQLVQD
IASLTLLEISDLNELLKKTLKIQDVGLVPMGGVMS
GAVPAAAAQEAVEEDIPIAKERTHFTVRLTEAKPV
DKVKLIKEIKNYIQGINLVQAKKLVESLPQEIKAN
VAKAEAEKIKAALEAVGGTVVLE- Isotype
- IgG
- Antibody clone number
- 3B12-1A3
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references A bifunctional protein regulates mitochondrial protein synthesis.
Oxygen consumption can regulate the growth of tumors, a new perspective on the Warburg effect.
Human mitochondrial ribosomal protein MRPL12 interacts directly with mitochondrial RNA polymerase to modulate mitochondrial gene expression.
Richman TR, Davies SM, Shearwood AM, Ermer JA, Scott LH, Hibbs ME, Rackham O, Filipovska A
Nucleic acids research 2014 May;42(9):5483-94
Nucleic acids research 2014 May;42(9):5483-94
Oxygen consumption can regulate the growth of tumors, a new perspective on the Warburg effect.
Chen Y, Cairns R, Papandreou I, Koong A, Denko NC
PloS one 2009 Sep 15;4(9):e7033
PloS one 2009 Sep 15;4(9):e7033
Human mitochondrial ribosomal protein MRPL12 interacts directly with mitochondrial RNA polymerase to modulate mitochondrial gene expression.
Wang Z, Cotney J, Shadel GS
The Journal of biological chemistry 2007 Apr 27;282(17):12610-8
The Journal of biological chemistry 2007 Apr 27;282(17):12610-8
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- MRPL12 monoclonal antibody (M01), clone 3B12-1A3 Western Blot analysis of MRPL12 expression in COLO 320 HSR ( Cat # L020V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of MRPL12 expression in transfected 293T cell line by MRPL12 monoclonal antibody (M01), clone 3B12-1A3.Lane 1: MRPL12 transfected lysate(21.3 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged MRPL12 is approximately 0.03ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to MRPL12 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to MRPL12 on formalin-fixed paraffin-embedded human breast cancer tissue. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
