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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00006182-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00006182-M01, RRID:AB_2145703
- Product name
- Mouse Monoclonal MRPL12 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. MRPL12 - mitochondrial ribosomal protein L12
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references A bifunctional protein regulates mitochondrial protein synthesis.
The mitochondrial RNA-binding protein GRSF1 localizes to RNA granules and is required for posttranscriptional mitochondrial gene expression.
Oxygen consumption can regulate the growth of tumors, a new perspective on the Warburg effect.
Human mitochondrial ribosomal protein MRPL12 interacts directly with mitochondrial RNA polymerase to modulate mitochondrial gene expression.
Richman TR, Davies SM, Shearwood AM, Ermer JA, Scott LH, Hibbs ME, Rackham O, Filipovska A
Nucleic acids research 2014 May;42(9):5483-94
Nucleic acids research 2014 May;42(9):5483-94
The mitochondrial RNA-binding protein GRSF1 localizes to RNA granules and is required for posttranscriptional mitochondrial gene expression.
Antonicka H, Sasarman F, Nishimura T, Paupe V, Shoubridge EA
Cell metabolism 2013 Mar 5;17(3):386-98
Cell metabolism 2013 Mar 5;17(3):386-98
Oxygen consumption can regulate the growth of tumors, a new perspective on the Warburg effect.
Chen Y, Cairns R, Papandreou I, Koong A, Denko NC
PloS one 2009 Sep 15;4(9):e7033
PloS one 2009 Sep 15;4(9):e7033
Human mitochondrial ribosomal protein MRPL12 interacts directly with mitochondrial RNA polymerase to modulate mitochondrial gene expression.
Wang Z, Cotney J, Shadel GS
The Journal of biological chemistry 2007 Apr 27;282(17):12610-8
The Journal of biological chemistry 2007 Apr 27;282(17):12610-8
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MRPL12 Antibody (3B12-1A3) [H00006182-M01] - Analysis of MRPL12 over-expressed 293 cell line, cotransfected with MRPL12 Validated Chimera RNAi ( Cat # H00006182-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MRPL12 monoclonal antibody (M01), clone 3B12-1A3 (Cat # H00006182-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MRPL12 Antibody (3B12-1A3) [H00006182-M01] - Analysis of MRPL12 expression in transfected 293T cell line by MRPL12 monoclonal antibody (M01), clone 3B12-1A3.Lane 1: MRPL12 transfected lysate(21.3 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MRPL12 Antibody (3B12-1A3) [H00006182-M01] - MRPL12 monoclonal antibody (M01), clone 3B12-1A3 Analysis of MRPL12 expression in COLO 320 HSR.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: MRPL12 Antibody (3B12-1A3) [H00006182-M01] - Detection limit for recombinant GST tagged MRPL12 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: MRPL12 Antibody (3B12-1A3) [H00006182-M01] - Analysis of monoclonal antibody to MRPL12 on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MRPL12 Antibody (3B12-1A3) [H00006182-M01] - Analysis of monoclonal antibody to MRPL12 on formalin-fixed paraffin-embedded human breast cancer tissue. Antibody concentration 3 ug/ml.
