Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-30159 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MRPL12 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, H1299, HeLa, HepG2, mouse brain.
- Concentration
- 0.56 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using MRPL12 Polyclonal Antibody (Product # PA5-30159). Sample (30 µg of whole cell lysate). Lane A: A431. 12% SDS PAGE. MRPL12 Polyclonal Antibody (Product # PA5-30159) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using MRPL12 Polyclonal Antibody (Product # PA5-30159). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 12% SDS PAGE. MRPL12 Polyclonal Antibody (Product # PA5-30159) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MRPL12 Polyclonal Antibody detects MRPL12 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min. Green: MRPL12 protein stained by MRPL12 Polyclonal Antibody (Product # PA5-30159) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer, using MRPL12 (Product # PA5-30159) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.