18284-1-AP

antibody from Proteintech Group

Targeting: HSPB1 CMT2F, Hs.76067, Hsp25, HSP27, HSP28

Provider product page for 18284-1-AP

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [37]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]

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Product number: 18284-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#18284-1-AP, RRID:AB_2295540
Product name: HSP27 antibody
Antibody type: Polyclonal
Description: HSP27 antibody (Cat. #18284-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, rat, mouse and has been validated for the following applications: FC, IF, IHC, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

HSPB1 protein structure - 18284-1-AP shown in red.

Submitted references Oxidative cyclization reagents reveal tryptophan cation-π interactions.

Xie X, Moon PJ, Crossley SWM, Bischoff AJ, He D, Li G, Dao N, Gonzalez-Valero A, Reeves AG, McKenna JM, Elledge SK, Wells JA, Toste FD, Chang CJ
Nature 2024 Mar;627(8004):680-687

5-AZA Upregulates SOCS3 and PTPN6/SHP1, Inhibiting STAT3 and Potentiating the Effects of AG490 against Primary Effusion Lymphoma Cells.

Di Crosta M, Arena A, Benedetti R, Gilardini Montani MS, Cirone M
Current issues in molecular biology 2024 Mar 14;46(3):2468-2479

Ginsenoside Rg5 inhibits glioblastoma by activating ferroptosis via NR3C1/HSPB1/NCOA4.

Zhang G, Hu J, Li A, Zhang H, Guo Z, Li X, You Z, Wang Y, Jing Z
Phytomedicine : international journal of phytotherapy and phytopharmacology 2024 Jul;129:155631

Phosphorylated heat shock protein 27 improves the bone formation ability of osteoblasts and bone marrow stem cells from patients with adolescent idiopathic scoliosis.

He S, Li J, Wang Y, Xiang G, Yang G, Xiao L, Tang M, Zhang H
JOR spine 2023 Sep;6(3):e1256

Analysis of proteome-wide degradation dynamics in ALS SOD1 iPSC-derived patient neurons reveals disrupted VCP homeostasis.

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Cell reports 2023 Oct 31;42(10):113160

FHOD1 is upregulated in glioma cells and attenuates ferroptosis of glioma cells by targeting HSPB1 signaling.

Zhang F, Wu L, Feng S, Zhao Z, Zhang K, Thakur A, Xu Z, Liang Q, Liu Y, Liu W, Yan Y
CNS neuroscience & therapeutics 2023 Nov;29(11):3351-3363

Human hematopoietic stem cell vulnerability to ferroptosis.

Zhao J, Jia Y, Mahmut D, Deik AA, Jeanfavre S, Clish CB, Sankaran VG
Cell 2023 Feb 16;186(4):732-747.e16

HSPs/STAT3 Interplay Sustains DDR and Promotes Cytokine Release by Primary Effusion Lymphoma Cells.

Gonnella R, Arena A, Zarrella R, Gilardini Montani MS, Santarelli R, Cirone M
International journal of molecular sciences 2023 Feb 15;24(4)

Enniatin A inhibits the chaperone Hsp90 and unleashes the immune system against triple-negative breast cancer.

Eisa NH, Crowley VM, Elahi A, Kommalapati VK, Serwetnyk MA, Llbiyi T, Lu S, Kainth K, Jilani Y, Marasco D, El Andaloussi A, Lee S, Tsai FTF, Rodriguez PC, Munn D, Celis E, Korkaya H, Debbab A, Blagg B, Chadli A
iScience 2023 Dec 15;26(12):108308

MiR-103a-3p Promotes Zika Virus Replication by Targeting OTU Deubiquitinase 4 to Activate p38 Mitogen-Activated Protein Kinase Signaling Pathway.

Ye H, Kang L, Yan X, Li S, Huang Y, Mu R, Duan X, Chen L
Frontiers in microbiology 2022;13:862580

FTH1- and SAT1-Induced Astrocytic Ferroptosis Is Involved in Alzheimer's Disease: Evidence from Single-Cell Transcriptomic Analysis.

Dang Y, He Q, Yang S, Sun H, Liu Y, Li W, Tang Y, Zheng Y, Wu T
Pharmaceuticals (Basel, Switzerland) 2022 Sep 22;15(10)

c-Myc Sustains Pancreatic Cancer Cell Survival and mutp53 Stability through the Mevalonate Pathway.

Romeo MA, Gilardini Montani MS, Arena A, Benedetti R, D'Orazi G, Cirone M
Biomedicines 2022 Oct 5;10(10)

Blood plasma derived extracellular vesicles (BEVs): particle purification liquid chromatography (PPLC) and proteomic analysis reveals BEVs as a potential minimally invasive tool for predicting response to breast cancer treatment.

Alvarez FA, Kaddour H, Lyu Y, Preece C, Cohen J, Baer L, Stopeck AT, Thompson P, Okeoma CM
Breast cancer research and treatment 2022 Nov;196(2):423-437

L-Theanine mediates the p38MAPK signaling pathway to alleviate heat-induced oxidative stress and inflammation in mice.

Liu K, Liu E, Lin L, Hu Y, Yuan Y, Xiao W
Food & function 2022 Feb 21;13(4):2120-2130

Construction and validation of a novel apoptosis-associated prognostic signature related to osteosarcoma metastasis and immune infiltration.

Fu Y, Jin Z, Shen Y, Zhang Z, Li M, Liu Z, He G, Wu J, Wen J, Bao Q, Wang J, Zhang W
Translational oncology 2022 Aug;22:101452

HSP27 Attenuates cGAS-Mediated IFN-β Signaling through Ubiquitination of cGAS and Promotes PRV Infection.

Li X, Xie J, Li D, Li H, Niu Y, Wu B, Yang Y, Yan Z, Zhang X, Chen L, Feng R
Viruses 2022 Aug 23;14(9)

HSP27 Protein Dampens Encephalomyocarditis Virus Replication by Stabilizing Melanoma Differentiation-Associated Gene 5.

Li X, Ma R, Wu B, Niu Y, Li H, Li D, Xie J, Idris A, Feng R
Frontiers in microbiology 2021;12:788870

ACE2 downregulation in olfactory mucosa: Eosinophilic rhinosinusitis as COVID-19 protective factor?

Marin C, Tubita V, Langdon C, Fuentes M, Rojas-Lechuga MJ, Valero A, Alobid I, Mullol J
Allergy 2021 Sep;76(9):2904-2907

Hu Y, Tu Z, Lei K, Huang K, Zhu X
Bioscience reports 2021 Dec 22;41(12)

Discovery of bladder cancer biomarkers in paired pre- and postoperative urine samples.

Li X, Huang C, Zhang X, Yang T, Zuo S, Fu C, Zhang Y, Yang C, Chen L
Translational andrology and urology 2021 Aug;10(8):3402-3414

Inhibitory effect of edible natural compounds with di- and tri-carboxyl moiety on endogenous protease inducing disassembly and degradation of myofibrils from grass carp (Ctenopharyngodon idella).

Ge L, Zhao N, Miao Y, Zhang S, Zhao M, Luo Y, Lai H, Huang Y, Wang Y
Food research international (Ottawa, Ont.) 2020 Nov;137:109457

Expression of the SARS-CoV-2 Entry Proteins, ACE2 and TMPRSS2, in Cells of the Olfactory Epithelium: Identification of Cell Types and Trends with Age.

Bilinska K, Jakubowska P, Von Bartheld CS, Butowt R
ACS chemical neuroscience 2020 Jun 3;11(11):1555-1562

Paclitaxel increases sensitivity of SKOV3 cells to hyperthermia by inhibiting heat shock protein 27.

Kong XX, Jiang S, Liu T, Liu GF, Dong M
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2020 Dec;132:110907

Rosmarinic acid exerts a neuroprotective effect on spinal cord injury by suppressing oxidative stress and inflammation via modulating the Nrf2/HO-1 and TLR4/NF-κB pathways.

Ma Z, Lu Y, Yang F, Li S, He X, Gao Y, Zhang G, Ren E, Wang Y, Kang X
Toxicology and applied pharmacology 2020 Apr 19;397:115014

Effects of Ethyl Pyruvate on Bile Duct Ligation-Induced Liver Fibrosis by Regulating Nrf2 Pathway and Proinflammatory Cytokines in Rats.

Zong Y, Zhang M, Li S, Qi W, Li J, Liu T, Yang H, Lu C, Hu X
Gastroenterology research and practice 2019;2019:2969802

Protein kinase D up-regulates transcription of VEGF receptor-2 in endothelial cells by suppressing nuclear localization of the transcription factor AP2β.

Wang Y, Hoeppner LH, Angom RS, Wang E, Dutta S, Doeppler HR, Wang F, Shen T, Scarisbrick IA, Guha S, Storz P, Bhattacharya R, Mukhopadhyay D
The Journal of biological chemistry 2019 Oct 25;294(43):15759-15767

Peroxiredoxin 6 Is a Crucial Factor in the Initial Step of Mitochondrial Clearance and Is Upstream of the PINK1-Parkin Pathway.

Ma S, Zhang X, Zheng L, Li Z, Zhao X, Lai W, Shen H, Lv J, Yang G, Wang Q, Ji J
Antioxidants & redox signaling 2016 Mar 20;24(9):486-501

Proteomic analysis of apoptosis induction by lariciresinol in human HepG2 cells.

Ma ZJ, Wang XX, Su G, Yang JJ, Zhu YJ, Wu YW, Li J, Lu L, Zeng L, Pei HX
Chemico-biological interactions 2016 Aug 25;256:209-19

Truncated HSPB1 causes axonal neuropathy and impairs tolerance to unfolded protein stress.

Ylikallio E, Konovalova S, Dhungana Y, Hilander T, Junna N, Partanen JV, Toppila JP, Auranen M, Tyynismaa H
BBA clinical 2015 Jun;3:233-42

Proteome analysis of porcine epidemic diarrhea virus (PEDV)-infected Vero cells.

Zeng S, Zhang H, Ding Z, Luo R, An K, Liu L, Bi J, Chen H, Xiao S, Fang L
Proteomics 2015 Jun;15(11):1819-28

Identification of heat shock protein 27 as a novel autoantigen of Behçet's disease.

Chen P, Shi L, Jiang Y, Ji Y, Yan H, Sun S, Xun Y, Chen G, Wang X, Chen W, Du H
Biochemical and biophysical research communications 2015 Jan 24;456(4):866-71

Identification of Hsf1 as a novel androgen receptor-regulated gene in mouse Sertoli cells.

Yang L, Wang Y, Zhang Q, Lai Y, Li C, Zhang Q, Huang W, Duan Y, Jiang Z, Li X, Cai Z, Mou L, Gui Y
Molecular reproduction and development 2014 Jun;81(6):514-23

Human myocytes are protected from titin aggregation-induced stiffening by small heat shock proteins.

Kötter S, Unger A, Hamdani N, Lang P, Vorgerd M, Nagel-Steger L, Linke WA
The Journal of cell biology 2014 Jan 20;204(2):187-202

Proteomic investigation of the sinulariolide-treated melanoma cells A375: effects on the cell apoptosis through mitochondrial-related pathway and activation of caspase cascade.

Li HH, Su JH, Chiu CC, Lin JJ, Yang ZY, Hwang WI, Chen YK, Lo YH, Wu YJ
Marine drugs 2013 Jul 22;11(7):2625-42

Upregulation of phosphorylated HSP27, PRDX2, GRP75, GRP78 and GRP94 in acquired middle ear cholesteatoma growth.

Ho KY, Yeh TS, Huang HH, Hung KF, Chai CY, Chen WT, Tsai SM, Chang NC, Chien CY, Wang HM, Wu YJ
International journal of molecular sciences 2013 Jul 11;14(7):14439-59

ATG4B/autophagin-1 regulates intestinal homeostasis and protects mice from experimental colitis.

Cabrera S, Fernández AF, Mariño G, Aguirre A, Suárez MF, Español Y, Vega JA, Laurà R, Fueyo A, Fernández-García MS, Freije JM, Kroemer G, López-Otín C
Autophagy 2013 Aug;9(8):1188-200

Proteomic investigation of anti-tumor activities exerted by sinularin against A2058 melanoma cells.

Su TR, Lin JJ, Chiu CC, Chen JY, Su JH, Cheng ZJ, Hwang WI, Huang HH, Wu YJ
Electrophoresis 2012 Apr;33(7):1139-52

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 18284-1-AP(HSPB1 antibody) at dilution of 1:500
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of MCF-7 cells, using HSPB1 antibody 18284-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line