Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP2-37599 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal HSP27 Antibody
- Antibody type
- Monoclonal
- Description
- Unpurified.
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HSP27 Antibody (5D7) [NBP2-37599] - Western blot analysis using HSP27 mouse mAb against Hela (1), A549 (2), Jurkat (3), A431 (4), HEK293(5), HepG2 (6) and PC-12 (7) cell lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: HSP27 Antibody (5D7) [NBP2-37599] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: HSP27 Antibody (5D7) [NBP2-37599] - Immunohistochemical analysis of paraffin-embedded brain tissues (left) and esophageal cancer tissues (right) using HSP27 mouse mAb with DAB staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: HSP27 Antibody (5D7) [NBP2-37599] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and cardiac muscle tissues (right) using HSP27 mouse mAb with DAB staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: HSP27 Antibody (5D7) [NBP2-37599] - Flow cytometric analysis of HepG2 cells using HSP27 mouse mAb (green) and negative control (purple).
