TA321366

antibody from OriGene

Targeting: HSPB1 CMT2F, Hs.76067, Hsp25, HSP27, HSP28

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA321366

Provider

OriGene

Product name

Anti-HSPB1 (Phospho-Ser15) Rabbit Polyclonal Antibody

Antibody type

Polyclonal

Description

Anti-HSPB1 (Phospho-Ser15) Rabbit Polyclonal Antibody

Host

Rabbit

Conjugate

Unconjugated

Epitope

HSPB1

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µl

Concentration

1mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted band size: 23 kDa . Positive control: Hela cells untreated or treated with UV lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1: Hela cells untreated with UV lysate Lane2: Hela cells treated with UV lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted cell location: Cytoplasm; Nucleus. Positive control: Hela cells. Recommended dilution: 1/ 100-200 The image is immunofluorescence of methanol-fixed Hela cells using HSPB1 (Phospho-Ser15) antibody at dilution 1/100. (Original magnification:?—200)

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted cell location: Cytoplasm; Nucleus. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using HSPB1 (Phospho-Ser15) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)

Validation comment

IHC