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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- TA337102 - Provider product page
- Provider
- OriGene
- Product name
- Mouse Monoclonal HSP27 Antibody (6H11)
- Antibody type
- Monoclonal
- Description
- Mouse Monoclonal HSP27 Antibody (6H11)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- HSPB1
- Isotype
- IgG
- Antibody clone number
- 6H11
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot: Hsp27 Antibody (6H11) [TA337102] - HeLa cell crude extracts. Lane 16 was probed with TA337102, while lanes 14 and 15 were probed with two other monoclonals to Hsp27. Note the strong clean bands at 27 kDa. Lane 17 was probed with NBP2-25151,
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Hsp27 Antibody (6H11) [TA337102] - HeLa cells stained with TA337102 (red), and counterstained with chicken polyclonal antibody to Vimentin NB300-223 (green) and DNA (blue). TA337102 reveals strong cytoplasmic staini
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow Cytometry: Hsp27 Antibody (6H11) [TA337102] - Flow cytometric staining of 1 x 10^6 MCF-7 cells using Hsp27 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
- Validation comment
- FC
