Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Immunohistochemistry [3]
- Other assay [1]
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Validation data
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- Product number
- V2134 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- HSP27 Antibody
- Antibody type
- Monoclonal
- Antigen
- Partially purified human HSP27 (earlier called 24K) from breast cancer MCF-7 cells was used as the immunogen for this antibody.
- Description
- Protein G purified antibody
- Reactivity
- Human, Mouse, Rat, Sheep
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- G3.1
- Vial size
- 20 µg, 100 µg (with sodium azide), 100 µg (without sodium azide), 7 ml (prediluted for IHC use, if applicable)
- Storage
- Store the HSP27 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Submitted references Fasting enhances TRAIL-mediated liver natural killer cell activity via HSP70 upregulation.
Effect of omeprazole dose, nonsteroidal anti-inflammatory agents, and smoking on repair mechanisms in acute peptic ulcer bleeding.
Inhibition of heat shock protein 27 phosphorylation promotes sensitivity to 5-fluorouracil in colorectal cancer cells.
Expression of heat-shock proteins hsp27, hsp70 and hsp90 in malignant epithelial tumour of the ovaries.
A single bout of eccentric exercise increases HSP27 and HSC/HSP70 in human skeletal muscle.
Intermediate filament interactions can be altered by HSP27 and alphaB-crystallin.
Heat shock protein 27 kDa expression and phosphorylation regulates endothelial cell migration.
HSP27 and HSP70 increase the survival of WEHI-S cells exposed to hyperthermia.
Dang VT, Tanabe K, Tanaka Y, Tokumoto N, Misumi T, Saeki Y, Fujikuni N, Ohdan H
PloS one 2014;9(10):e110748
PloS one 2014;9(10):e110748
Effect of omeprazole dose, nonsteroidal anti-inflammatory agents, and smoking on repair mechanisms in acute peptic ulcer bleeding.
Rantanen T, Udd M, Honkanen T, Miettinen P, Kärjä V, Rantanen L, Julkunen R, Mustonen H, Paavonen T, Oksala N
Digestive diseases and sciences 2014 Nov;59(11):2666-74
Digestive diseases and sciences 2014 Nov;59(11):2666-74
Inhibition of heat shock protein 27 phosphorylation promotes sensitivity to 5-fluorouracil in colorectal cancer cells.
Matsunaga A, Ishii Y, Tsuruta M, Okabayashi K, Hasegawa H, Kitagawa Y
Oncology letters 2014 Dec;8(6):2496-2500
Oncology letters 2014 Dec;8(6):2496-2500
Expression of heat-shock proteins hsp27, hsp70 and hsp90 in malignant epithelial tumour of the ovaries.
Elpek GO, Karaveli S, Simşek T, Keles N, Aksoy NH
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2003 Apr;111(4):523-30
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2003 Apr;111(4):523-30
A single bout of eccentric exercise increases HSP27 and HSC/HSP70 in human skeletal muscle.
Thompson HS, Scordilis SP, Clarkson PM, Lohrer WA
Acta physiologica Scandinavica 2001 Feb;171(2):187-93
Acta physiologica Scandinavica 2001 Feb;171(2):187-93
Intermediate filament interactions can be altered by HSP27 and alphaB-crystallin.
Perng MD, Cairns L, van den IJssel P, Prescott A, Hutcheson AM, Quinlan RA
Journal of cell science 1999 Jul;112 ( Pt 13):2099-112
Journal of cell science 1999 Jul;112 ( Pt 13):2099-112
Heat shock protein 27 kDa expression and phosphorylation regulates endothelial cell migration.
Piotrowicz RS, Hickey E, Levin EG
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 1998 Nov;12(14):1481-90
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 1998 Nov;12(14):1481-90
HSP27 and HSP70 increase the survival of WEHI-S cells exposed to hyperthermia.
Wissing D, Jäättelä M
International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group 1996 Jan-Feb;12(1):125-38
International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group 1996 Jan-Feb;12(1):125-38
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of human breast carcinoma (10X) stained with HSP27 antibody (G3.1).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of breast carcinoma (20X) stained with HSP27 antibody (G3.1).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human prostate carcinoma and HSP27 antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE Analysis of Purified, BSA-Free HSP27 Antibody (clone G3.1). Confirmation of Integrity and Purity of the Antibody.