V2134

antibody from NSJ Bioreagents

Targeting: HSPB1 CMT2F, Hs.76067, Hsp25, HSP27, HSP28

Western blot (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

V2134

Provider

NSJ Bioreagents

Product name

HSP27 Antibody

Antibody type

Monoclonal

Antigen

Partially purified human HSP27 (earlier called 24K) from breast cancer MCF-7 cells was used as the immunogen for this antibody.

Description

Protein G purified antibody

Reactivity

Human, Mouse, Rat, Sheep

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

G3.1

Vial size

20 µg, 100 µg (with sodium azide), 100 µg (without sodium azide), 7 ml (prediluted for IHC use, if applicable)

Storage

Store the HSP27 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

References

Submitted references

Fasting enhances TRAIL-mediated liver natural killer cell activity via HSP70 upregulation.

Dang VT, Tanabe K, Tanaka Y, Tokumoto N, Misumi T, Saeki Y, Fujikuni N, Ohdan H
PloS one 2014;9(10):e110748

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Effect of omeprazole dose, nonsteroidal anti-inflammatory agents, and smoking on repair mechanisms in acute peptic ulcer bleeding.

Rantanen T, Udd M, Honkanen T, Miettinen P, Kärjä V, Rantanen L, Julkunen R, Mustonen H, Paavonen T, Oksala N
Digestive diseases and sciences 2014 Nov;59(11):2666-74

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Inhibition of heat shock protein 27 phosphorylation promotes sensitivity to 5-fluorouracil in colorectal cancer cells.

Matsunaga A, Ishii Y, Tsuruta M, Okabayashi K, Hasegawa H, Kitagawa Y
Oncology letters 2014 Dec;8(6):2496-2500

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Expression of heat-shock proteins hsp27, hsp70 and hsp90 in malignant epithelial tumour of the ovaries.

Elpek GO, Karaveli S, Simşek T, Keles N, Aksoy NH
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2003 Apr;111(4):523-30

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A single bout of eccentric exercise increases HSP27 and HSC/HSP70 in human skeletal muscle.

Thompson HS, Scordilis SP, Clarkson PM, Lohrer WA
Acta physiologica Scandinavica 2001 Feb;171(2):187-93

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Intermediate filament interactions can be altered by HSP27 and alphaB-crystallin.

Perng MD, Cairns L, van den IJssel P, Prescott A, Hutcheson AM, Quinlan RA
Journal of cell science 1999 Jul;112 ( Pt 13):2099-112

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Heat shock protein 27 kDa expression and phosphorylation regulates endothelial cell migration.

Piotrowicz RS, Hickey E, Levin EG
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 1998 Nov;12(14):1481-90

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HSP27 and HSP70 increase the survival of WEHI-S cells exposed to hyperthermia.

Wissing D, Jäättelä M
International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group 1996 Jan-Feb;12(1):125-38

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of human breast carcinoma (10X) stained with HSP27 antibody (G3.1).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of breast carcinoma (20X) stained with HSP27 antibody (G3.1).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human prostate carcinoma and HSP27 antibody.

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE Analysis of Purified, BSA-Free HSP27 Antibody (clone G3.1). Confirmation of Integrity and Purity of the Antibody.