AM00070PU-N

antibody from OriGene

Targeting: HSPB1 CMT2F, Hs.76067, Hsp25, HSP27, HSP28

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

AM00070PU-N

Provider

OriGene

Product name

HSP27 (HSPB1) pSer82 (incl. pos. control) mouse monoclonal antibody, clone 5B9, Purified

Antibody type

Monoclonal

Description

HSP27 (HSPB1) pSer82 (incl. pos. control) mouse monoclonal antibody, clone 5B9, Purified

Host

Mouse

Conjugate

Unconjugated

Epitope

HSPB1

Isotype

IgG

Antibody clone number

5B9

Vial size

100 µg

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HSP 27 activation Serum starved HepG2 cells were incubated with 10 ng/ml EGF for the indicated times. Whole cell lysates were prepared with lysis buffer V19 and separated by SDS-PAGE (ca 20.000 cells/lane). Immunoblots were probed with mab hsp27-5B9 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: Control Lane 2: 5 min EGF Lane 3: 15 min EGF Lane 4: 30 min EGF Lane 5: 1h EGF Lane 6; 2h EGF Lane 7: 4h EGF Lane 8: 8h EGF

Validation comment

WB