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- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- AM09016PU-S - Provider product page
- Provider
- OriGene
- Product name
- HSP27 (HSPB1) mouse monoclonal antibody, clone 2A5, Purified
- Antibody type
- Monoclonal
- Description
- HSP27 (HSPB1) mouse monoclonal antibody, clone 2A5, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- HSPB1
- Isotype
- IgG
- Antibody clone number
- 2A5
- Vial size
- 50 µl
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis on HeLa cells: Cell lysates of HeLa (each 30 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hsp27 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Cell lysates of HeLa(each 30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hsp27 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Paraffin embedded sections of human colon cancer tissue were incubated with anti-human Hsp27 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry : Paraffin embedded sections of human colon cancer tissue were incubated with anti-human Hsp27 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and Diaminobenzidine (DAB) was used for detection.
- Validation comment
- IHC
