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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- AM06625SU-N - Provider product page
- Provider
- OriGene
- Product name
- HSP27 (HSPB1) mouse monoclonal antibody, clone 5D7, Ascites
- Antibody type
- Monoclonal
- Description
- HSP27 (HSPB1) mouse monoclonal antibody, clone 5D7, Ascites
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- HSPB1
- Isotype
- IgG
- Antibody clone number
- 5D7
- Vial size
- 100 µl
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis using HSP27 mouse mAb against Hela (1), A549 (2), Jurkat (3), A431 (4), HEK293(5), HepG2 (6) and PC-12 (7) cell lysate.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
- Validation comment
- ELISA
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Hela cells using HSP27 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and cardiac muscle tissues (right) using HSP27 mouse mAb with DAB staining.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded brain tissues (left) and esophageal cancer tissues (right) using HSP27 mouse mAb with DAB staining.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HepG2 cells using HSP27 mouse mAb (green) and negative control (purple).
- Validation comment
- FC
