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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PAB0490 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0490, RRID:AB_982615
- Product name
- HSPB1 (phospho S78) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of HSPB1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Mutant small heat-shock protein 27 causes axonal Charcot-Marie-Tooth disease and distal hereditary motor neuropathy.
Stat3 modulates heat shock 27kDa protein expression in breast epithelial cells.
Protective role of HSP27 against UVC-induced cell death in human cells.
Evgrafov OV, Mersiyanova I, Irobi J, Van Den Bosch L, Dierick I, Leung CL, Schagina O, Verpoorten N, Van Impe K, Fedotov V, Dadali E, Auer-Grumbach M, Windpassinger C, Wagner K, Mitrovic Z, Hilton-Jones D, Talbot K, Martin JJ, Vasserman N, Tverskaya S, Polyakov A, Liem RK, Gettemans J, Robberecht W, De Jonghe P, Timmerman V
Nature genetics 2004 Jun;36(6):602-6
Nature genetics 2004 Jun;36(6):602-6
Stat3 modulates heat shock 27kDa protein expression in breast epithelial cells.
Song H, Ethier SP, Dziubinski ML, Lin J
Biochemical and biophysical research communications 2004 Jan 30;314(1):143-50
Biochemical and biophysical research communications 2004 Jan 30;314(1):143-50
Protective role of HSP27 against UVC-induced cell death in human cells.
Wano C, Kita K, Takahashi S, Sugaya S, Hino M, Hosoya H, Suzuki N
Experimental cell research 2004 Aug 15;298(2):584-92
Experimental cell research 2004 Aug 15;298(2):584-92
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- HSPB1 (phospho S78) polyclonal antibody (Cat # PAB0490). Western blot analysis of HSPB1 expression in human stomach.
- Validation comment
- Western Blot (Tissue lysate)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Dot blot analysis of HSPB1 (phospho S78) polyclonal antibody (Cat # PAB0490) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5 ug/mL. P-Pab : phospho-antibody; P-Peptide : phospho-peptide; NP-Peptide : non-phospho-peptide.
- Validation comment
- Dot Blot (Peptide)
