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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB2047 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2047, RRID:AB_1576103
- Product name
- HSPB1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of HSPB1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Mutant small heat-shock protein 27 causes axonal Charcot-Marie-Tooth disease and distal hereditary motor neuropathy.
Stat3 modulates heat shock 27kDa protein expression in breast epithelial cells.
Protective role of HSP27 against UVC-induced cell death in human cells.
Evgrafov OV, Mersiyanova I, Irobi J, Van Den Bosch L, Dierick I, Leung CL, Schagina O, Verpoorten N, Van Impe K, Fedotov V, Dadali E, Auer-Grumbach M, Windpassinger C, Wagner K, Mitrovic Z, Hilton-Jones D, Talbot K, Martin JJ, Vasserman N, Tverskaya S, Polyakov A, Liem RK, Gettemans J, Robberecht W, De Jonghe P, Timmerman V
Nature genetics 2004 Jun;36(6):602-6
Nature genetics 2004 Jun;36(6):602-6
Stat3 modulates heat shock 27kDa protein expression in breast epithelial cells.
Song H, Ethier SP, Dziubinski ML, Lin J
Biochemical and biophysical research communications 2004 Jan 30;314(1):143-50
Biochemical and biophysical research communications 2004 Jan 30;314(1):143-50
Protective role of HSP27 against UVC-induced cell death in human cells.
Wano C, Kita K, Takahashi S, Sugaya S, Hino M, Hosoya H, Suzuki N
Experimental cell research 2004 Aug 15;298(2):584-92
Experimental cell research 2004 Aug 15;298(2):584-92
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of HSPB1 (arrow) using HSPB1 polyclonal antibody (Cat # PAB2047). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HSPB1 gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with HSPB1 polyclonal antibody (Cat # PAB2047) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
