Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB18476 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB18476, RRID:AB_10716836
- Product name
- HSPB1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of HSPB1.
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references CXCL12 induces tyrosine phosphorylation of cortactin, which plays a role in CXC chemokine receptor 4-mediated extracellular signal-regulated kinase activation and chemotaxis.
Characterization of heat shock protein 27 phosphorylation sites in renal cell carcinoma.
F-actin capping (CapZ) and other contractile saphenous vein smooth muscle proteins are altered by hemodynamic stress: a proteonomic approach.
Cortactin tyrosine phosphorylation requires Rac1 activity and association with the cortical actin cytoskeleton.
Hsp27 upregulation and phosphorylation is required for injured sensory and motor neuron survival.
Tyrosine phosphorylation of Kv1.2 modulates its interaction with the actin-binding protein cortactin.
A strategy for the rapid identification of phosphorylation sites in the phosphoproteome.
Luo C, Pan H, Mines M, Watson K, Zhang J, Fan GH
The Journal of biological chemistry 2006 Oct 6;281(40):30081-93
The Journal of biological chemistry 2006 Oct 6;281(40):30081-93
Characterization of heat shock protein 27 phosphorylation sites in renal cell carcinoma.
Tremolada L, Magni F, Valsecchi C, Sarto C, Mocarelli P, Perego R, Cordani N, Favini P, Galli Kienle M, Sanchez JC, Hochstrasser DF, Corthals GL
Proteomics 2005 Feb;5(3):788-95
Proteomics 2005 Feb;5(3):788-95
F-actin capping (CapZ) and other contractile saphenous vein smooth muscle proteins are altered by hemodynamic stress: a proteonomic approach.
McGregor E, Kempster L, Wait R, Gosling M, Dunn MJ, Powell JT
Molecular & cellular proteomics : MCP 2004 Feb;3(2):115-24
Molecular & cellular proteomics : MCP 2004 Feb;3(2):115-24
Cortactin tyrosine phosphorylation requires Rac1 activity and association with the cortical actin cytoskeleton.
Head JA, Jiang D, Li M, Zorn LJ, Schaefer EM, Parsons JT, Weed SA
Molecular biology of the cell 2003 Aug;14(8):3216-29
Molecular biology of the cell 2003 Aug;14(8):3216-29
Hsp27 upregulation and phosphorylation is required for injured sensory and motor neuron survival.
Benn SC, Perrelet D, Kato AC, Scholz J, Decosterd I, Mannion RJ, Bakowska JC, Woolf CJ
Neuron 2002 Sep 26;36(1):45-56
Neuron 2002 Sep 26;36(1):45-56
Tyrosine phosphorylation of Kv1.2 modulates its interaction with the actin-binding protein cortactin.
Hattan D, Nesti E, Cachero TG, Morielli AD
The Journal of biological chemistry 2002 Oct 11;277(41):38596-606
The Journal of biological chemistry 2002 Oct 11;277(41):38596-606
A strategy for the rapid identification of phosphorylation sites in the phosphoproteome.
MacDonald JA, Mackey AJ, Pearson WR, Haystead TA
Molecular & cellular proteomics : MCP 2002 Apr;1(4):314-22
Molecular & cellular proteomics : MCP 2002 Apr;1(4):314-22
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from HeLa cells using HSPB1 polyclonal antibody (Cat # PAB18476).Peptide "+" means "peptide blocking".
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using HSPB1 polyclonal antibody (Cat # PAB18476).Peptide "+" means "peptide blocking".
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)