711351

antibody from Invitrogen Antibodies

Targeting: HSPB1 CMT2F, Hs.76067, Hsp25, HSP27, HSP28

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

711351

Provider

Invitrogen Antibodies

Product name

Phospho-HSP27 (Ser78) Recombinant Polyclonal Antibody (4HCLC)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with Monkey, Dog, Cat, Pig

Antibody clone number

4HCLC

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on Whole cell extracts (30 µg lysate) of HeLa, HeLa (UV for 40 min). The blots were probed with Anti-Hsp27 (pS78) Recombinant Rabbit Polyclonal Antibody (Product # 711351, 1-2 µg/mL) (Lane 1, 2). To confirm the specificity of Anti-Anti-Hsp27 (pS78), competition was performed with the phospopeptide (10 µg/mL) (Lane 3, 4) and non phospopeptide (10 µg/mL) (Lane 5, 6). A 27 kDa band corresponding to Anti-Hsp27 (pS78) was observed in HeLa (with increase in expression upon UV treatment) when probed with the antibody alone or with non-phospho peptide competition. The blots were detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).