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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- ABIN359390 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Heat Shock 27kDa Protein 1 (HSPB1) (Ser82) antibody
- Antibody type
- Polyclonal
- Antigen
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S82 of human HSPB1.
- Description
- Protein A column, followed by peptide affinity purification
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- Ser82
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store the antibody at 2 - 8°C up to one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Mutant small heat-shock protein 27 causes axonal Charcot-Marie-Tooth disease and distal hereditary motor neuropathy.
Stat3 modulates heat shock 27kDa protein expression in breast epithelial cells.
Protective role of HSP27 against UVC-induced cell death in human cells.
Hsp27 inhibits release of mitochondrial protein Smac in multiple myeloma cells and confers dexamethasone resistance.
Hsp27 associates with actin and limits injury in energy depleted renal epithelia.
Evgrafov OV, Mersiyanova I, Irobi J, Van Den Bosch L, Dierick I, Leung CL, Schagina O, Verpoorten N, Van Impe K, Fedotov V, Dadali E, Auer-Grumbach M, Windpassinger C, Wagner K, Mitrovic Z, Hilton-Jones D, Talbot K, Martin JJ, Vasserman N, Tverskaya S, Polyakov A, Liem RK, Gettemans J, Robberecht W, De Jonghe P, Timmerman V
Nature genetics 2004 Jun;36(6):602-6
Nature genetics 2004 Jun;36(6):602-6
Stat3 modulates heat shock 27kDa protein expression in breast epithelial cells.
Song H, Ethier SP, Dziubinski ML, Lin J
Biochemical and biophysical research communications 2004 Jan 30;314(1):143-50
Biochemical and biophysical research communications 2004 Jan 30;314(1):143-50
Protective role of HSP27 against UVC-induced cell death in human cells.
Wano C, Kita K, Takahashi S, Sugaya S, Hino M, Hosoya H, Suzuki N
Experimental cell research 2004 Aug 15;298(2):584-92
Experimental cell research 2004 Aug 15;298(2):584-92
Hsp27 inhibits release of mitochondrial protein Smac in multiple myeloma cells and confers dexamethasone resistance.
Chauhan D, Li G, Hideshima T, Podar K, Mitsiades C, Mitsiades N, Catley L, Tai YT, Hayashi T, Shringarpure R, Burger R, Munshi N, Ohtake Y, Saxena S, Anderson KC
Blood 2003 Nov 1;102(9):3379-86
Blood 2003 Nov 1;102(9):3379-86
Hsp27 associates with actin and limits injury in energy depleted renal epithelia.
Van Why SK, Mann AS, Ardito T, Thulin G, Ferris S, Macleod MA, Kashgarian M, Siegel NJ
Journal of the American Society of Nephrology : JASN 2003 Jan;14(1):98-106
Journal of the American Society of Nephrology : JASN 2003 Jan;14(1):98-106
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of HSPB1 (arrow) using rabbit polyclonal HSPB1 Antibody. 293 cell lysates (2 μg/lane) either nontransfected (Lane 1) or transiently transfected with the HSPB1 gene (Lane 2)
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with HSPB1 Antibody (AP13874PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
