H00003315-M04

antibody from Novus Biologicals

Targeting: HSPB1 CMT2F, Hs.76067, Hsp25, HSP27, HSP28

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Proximity ligation assay (Data presented on provider website)

Antibody Data

Product number

H00003315-M04

Provider

Novus Biologicals

Proper citation

Novus Cat#H00003315-M04, RRID:AB_790278

Product name

Mouse Monoclonal HSP27 Antibody

Antibody type

Monoclonal

Description

IgG purified. HSPB1 (3G3)

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: HSP27 Antibody (3G3) [H00003315-M04] - Analysis of HSPB1 expression in transfected 293T cell line by HSPB1 monoclonal antibody (M04), clone 3G3. Lane 1: HSPB1 transfected lysatE (22.8 KDa). Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

ELISA: HSP27 Antibody (3G3) [H00003315-M04] - Detection limit for recombinant GST tagged HSPB1 is approximately 0.1ng/ml as a capture antibody.

Immunocytochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence: HSP27 Antibody (3G3) [H00003315-M04] - Analysis of monoclonal antibody to HSPB1 on HeLa cell. Antibody concentration 10 ug/ml

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: HSP27 Antibody (3G3) [H00003315-M04] - Analysis of HSPB1 transfected lysate using anti-HSPB1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HSPB1 rabbit polyclonal antibody.