MA5-41052

antibody from Invitrogen Antibodies

Targeting: HSPB1 CMT2F, Hs.76067, Hsp25, HSP27, HSP28

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-41052

Provider

Invitrogen Antibodies

Product name

HSP27 Recombinant Rabbit Monoclonal Antibody (017)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

017

Vial size

100 µL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of HSP27 in Lane A: 0.5 mg Caco-2 Whole Cell Lysate. Samples were treated with 15 μl of 50 % Protein G agarose, incubated with monoclonal antibody (Product # MA5-41052) with a dilution of 1:500 , followed by Dylight 800-labeled antibody to rabbit IgG (H+L) using a dilution of 1:5,000. Assay was performed under reducing conditions. Predicted band size: 25 kDa, Observed band size: 25 kDa .

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HSP27 Immunoprecipitation using: Lane A: 0.5 mg Caco-2 Whole Cell Lysate 2 µL with HSP27 Recombinant Rabbit Monoclonal Antibody (017) (Product # MA5-41052) and 15 µL of 50 % Protein G agarose. Primary antibody: HSP27 Recombinant Rabbit Monoclonal Antibody (017), at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 25 kDa. Observed band size: 25 kDa.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of HSP27 in Lane A: 0.5 mg Caco-2 Whole Cell Lysate. Samples were treated with 15 μl of 50 % Protein G agarose, incubated with monoclonal antibody (Product # MA5-41052) with a dilution of 1:500 , followed by Dylight 800-labeled antibody to rabbit IgG (H+L) using a dilution of 1:5,000. Assay was performed under reducing conditions. Predicted band size: 25 kDa, Observed band size: 25 kDa .

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HSP27 Immunoprecipitation using: Lane A: 0.5 mg Caco-2 Whole Cell Lysate 2 µL with HSP27 Recombinant Rabbit Monoclonal Antibody (017) (Product # MA5-41052) and 15 µL of 50 % Protein G agarose. Primary antibody: HSP27 Recombinant Rabbit Monoclonal Antibody (017), at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 25 kDa. Observed band size: 25 kDa.