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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [4]
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- Product number
- NBP1-89666 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-89666, RRID:AB_11003492
- Product name
- Rabbit Polyclonal HSP27 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human HSP27 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references From gene expression analysis to tissue microarrays: a rational approach to identify therapeutic and diagnostic targets in lymphoid malignancies.
Ek S, Andréasson U, Hober S, Kampf C, Pontén F, Uhlén M, Merz H, Borrebaeck CA
Molecular & cellular proteomics : MCP 2006 Jun;5(6):1072-81
Molecular & cellular proteomics : MCP 2006 Jun;5(6):1072-81
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HSP27 Antibody [NBP1-89666] - Analysis in human cell lines MCF-7 and Caco-2 using anti-HSPB1 antibody. Corresponding HSPB1 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HSP27 Antibody [NBP1-89666] - Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HSP27 Antibody [NBP1-89666] - Staining of human pancreas shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HSP27 Antibody [NBP1-89666] - Staining of human skin shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HSP27 Antibody [NBP1-89666] - Staining in human skin and pancreas tissues using anti-HSPB1 antibody. Corresponding HSPB1 RNA-seq data are presented for the same tissues.
