AM00070PU-N

antibody from Acris Antibodies GmbH

Targeting: HSPB1 CMT2F, Hs.76067, Hsp25, HSP27, HSP28

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

AM00070PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM00070PU-N, RRID:AB_1002562

Product name

anti HSPB1 / HSP27 pSer82 (incl. pos. control)

Antibody type

Monoclonal

Antigen

Synthetic phosphopeptide conjugated to KLH.Epitope: R Q L pS S G V

Reactivity

Human, Mouse, Rat, Canine

Host

Mouse

Isotype

IgG

Antibody clone number

5B9

Vial size

0.1 mg

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

HSP 27 activation Serum starved HepG2 cells were incubated with 10 ng/ml EGF for the indicated times. Whole cell lysates were prepared with lysis buffer V19 and separated by SDS-PAGE (ca 20.000 cells/lane). Immunoblots were probed with mab hsp27-5B9 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: Control Lane 2: 5 min EGF Lane 3: 15 min EGF Lane 4: 30 min EGF Lane 5: 1h EGFLane 6; 2h EGF Lane 7: 4h EGFLane 8: 8h EGF