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LearnAP21899PU-N
antibody from Acris Antibodies GmbH
Targeting: HSPB1
CMT2F, Hs.76067, Hsp25, HSP27, HSP28
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AP21899PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP21899PU-N, RRID:AB_10778741
- Product name
- anti HSPB1 / HSP27
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 50-100 of human HSP27
- Reactivity
- Human, Canine, Simian
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of HSP27 in human skeletal muscle lysate using 1) preimmune sera at 1/5000 and 2) peptide affinity purified AP21899PU-N at 2.0 µg/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human small intestine probed with HSP27 antibody (Cat.-No.:AP21899PU-N) at 10 µg/ml.Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
