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antibody from GeneTex
Targeting: NDC80
HEC, HEC1, hsNDC80, KNTC2, TID3
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Proximity ligation assayAntibody data
- Antibody Data
- Antigen structure
- References [64]
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- Validations
- Western blot [6]
- Immunocytochemistry [4]
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- Product number
- GTX70268 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX70268, RRID:AB_371632
- Product name
- Hec1 antibody [9G3.23]
- Antibody type
- Monoclonal
- Reactivity
- Human, Hamster
- Host
- Mouse
Submitted references KIF18A's neck linker permits navigation of microtubule-bound obstacles within the mitotic spindle.
CDK1-CCNB1 creates a spindle checkpoint-permissive state by enabling MPS1 kinetochore localization.
MAD1-dependent recruitment of CDK1-CCNB1 to kinetochores promotes spindle checkpoint signaling.
HJURP antagonizes CENP-A mislocalization driven by the H3.3 chaperones HIRA and DAXX.
Loss of Kif18A Results in Spindle Assembly Checkpoint Activation at Microtubule-Attached Kinetochores.
Local activation of mammalian separase in interphase promotes double-strand break repair and prevents oncogenic transformation.
The ESCRT protein Chmp4c regulates mitotic spindle checkpoint signaling.
Studying meiotic cohesin in somatic cells reveals that Rec8-containing cohesin requires Stag3 to function and is regulated by Wapl and sororin.
Chmp4c is required for stable kinetochore-microtubule attachments.
Role of Kif15 and its novel mitotic partner KBP in K-fiber dynamics and chromosome alignment.
The E2F activators control multiple mitotic regulators and maintain genomic integrity through Sgo1 and BubR1.
SGT1-HSP90 complex is required for CENP-A deposition at centromeres.
The tobacco carcinogen NNK disturbs mitotic chromosome alignment by interrupting p53 targeting to the centrosome.
Mechanism of Ska Recruitment by Ndc80 Complexes to Kinetochores.
Spindle assembly checkpoint signaling and sister chromatid cohesion are disrupted by HPV E6-mediated transformation.
Measuring Kinetochore-Microtubule Attachment Stability in Cultured Cells.
The Ska complex promotes Aurora B activity to ensure chromosome biorientation.
Structure of the MIS12 Complex and Molecular Basis of Its Interaction with CENP-C at Human Kinetochores.
Preclinical activity of MBM-5 in gastrointestinal cancer by inhibiting NEK2 kinase activity.
Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore targeting of Mad1-Mad2.
Molecular basis of outer kinetochore assembly on CENP-T.
Phosphorylation of Astrin Regulates Its Kinetochore Function.
Mitotic Protein CSPP1 Interacts with CENP-H Protein to Coordinate Accurate Chromosome Oscillation in Mitosis.
Requirement for PLK1 kinase activity in the maintenance of a robust spindle assembly checkpoint.
Stable kinetochore-microtubule attachment is sufficient to silence the spindle assembly checkpoint in human cells.
CENP-W plays a role in maintaining bipolar spindle structure.
Inhibition of Hec1 as a novel approach for treatment of primary liver cancer.
PP2A-B56 opposes Mps1 phosphorylation of Knl1 and thereby promotes spindle assembly checkpoint silencing.
CENP-I and Aurora B act as a molecular switch that ties RZZ/Mad1 recruitment to kinetochore attachment status.
Chk2 prevents mitotic exit when the majority of kinetochores are unattached.
Activity of a novel Hec1-targeted anticancer compound against breast cancer cell lines in vitro and in vivo.
The pseudo GTPase CENP-M drives human kinetochore assembly.
Pre-anaphase chromosome oscillations are regulated by the antagonistic activities of Cdk1 and PP1 on Kif18A.
Prophase pathway-dependent removal of cohesin from human chromosomes requires opening of the Smc3-Scc1 gate.
KNL1 facilitates phosphorylation of outer kinetochore proteins by promoting Aurora B kinase activity.
Ubiquitylation-dependent localization of PLK1 in mitosis.
A small-molecule inhibitor of Haspin alters the kinetochore functions of Aurora B.
Subdiffraction imaging of centrosomes reveals higher-order organizational features of pericentriolar material.
Aurora B controls kinetochore-microtubule attachments by inhibiting Ska complex-KMN network interaction.
A conserved role for COMA/CENP-H/I/N kinetochore proteins in the spindle checkpoint.
Structural analysis reveals features of the spindle checkpoint kinase Bub1-kinetochore subunit Knl1 interaction.
Phosphorylation of the Ndc80 complex protein, HEC1, by Nek2 kinase modulates chromosome alignment and signaling of the spindle assembly checkpoint.
Small GTPase Rab5 participates in chromosome congression and regulates localization of the centromere-associated protein CENP-F to kinetochores.
Direct binding of Cenp-C to the Mis12 complex joins the inner and outer kinetochore.
The NDC80 complex proteins Nuf2 and Hec1 make distinct contributions to kinetochore-microtubule attachment in mitosis.
HJURP is a CENP-A chromatin assembly factor sufficient to form a functional de novo kinetochore.
Evidence that Aurora B is implicated in spindle checkpoint signalling independently of error correction.
The Ndc80 complex uses a tripartite attachment point to couple microtubule depolymerization to chromosome movement.
The MIS12 complex is a protein interaction hub for outer kinetochore assembly.
A screen for kinetochore-microtubule interaction inhibitors identifies novel antitubulin compounds.
Dissecting the role of MPS1 in chromosome biorientation and the spindle checkpoint through the small molecule inhibitor reversine.
Protein phosphatase 6 regulates mitotic spindle formation by controlling the T-loop phosphorylation state of Aurora A bound to its activator TPX2.
A mitosis block links active cell cycle with human epidermal differentiation and results in endoreplication.
Hec1 contributes to mitotic centrosomal microtubule growth for proper spindle assembly through interaction with Hice1.
Mitotic control of kinetochore-associated dynein and spindle orientation by human Spindly.
Small molecule targeting the Hec1/Nek2 mitotic pathway suppresses tumor cell growth in culture and in animal.
Kinetochore attachments require an interaction between unstructured tails on microtubules and Ndc80(Hec1).
Aurora A regulates the activity of HURP by controlling the accessibility of its microtubule-binding domain.
MCAK-independent functions of ch-Tog/XMAP215 in microtubule plus-end dynamics.
FAM29A promotes microtubule amplification via recruitment of the NEDD1-gamma-tubulin complex to the mitotic spindle.
DDA3 recruits microtubule depolymerase Kif2a to spindle poles and controls spindle dynamics and mitotic chromosome movement.
PRP4 is a spindle assembly checkpoint protein required for MPS1, MAD1, and MAD2 localization to the kinetochores.
Centromere targeting of the chromosomal passenger complex requires a ternary subcomplex of Borealin, Survivin, and the N-terminal domain of INCENP.
HURP controls spindle dynamics to promote proper interkinetochore tension and efficient kinetochore capture.
Malaby HL, Lessard DV, Berger CL, Stumpff J
Life science alliance 2019 Feb;2(1)
Life science alliance 2019 Feb;2(1)
CDK1-CCNB1 creates a spindle checkpoint-permissive state by enabling MPS1 kinetochore localization.
Hayward D, Alfonso-Pérez T, Cundell MJ, Hopkins M, Holder J, Bancroft J, Hutter LH, Novak B, Barr FA, Gruneberg U
The Journal of cell biology 2019 Apr 1;218(4):1182-1199
The Journal of cell biology 2019 Apr 1;218(4):1182-1199
MAD1-dependent recruitment of CDK1-CCNB1 to kinetochores promotes spindle checkpoint signaling.
Alfonso-Pérez T, Hayward D, Holder J, Gruneberg U, Barr FA
The Journal of cell biology 2019 Apr 1;218(4):1108-1117
The Journal of cell biology 2019 Apr 1;218(4):1108-1117
HJURP antagonizes CENP-A mislocalization driven by the H3.3 chaperones HIRA and DAXX.
Nye J, Sturgill D, Athwal R, Dalal Y
PloS one 2018;13(10):e0205948
PloS one 2018;13(10):e0205948
Loss of Kif18A Results in Spindle Assembly Checkpoint Activation at Microtubule-Attached Kinetochores.
Janssen LME, Averink TV, Blomen VA, Brummelkamp TR, Medema RH, Raaijmakers JA
Current biology : CB 2018 Sep 10;28(17):2685-2696.e4
Current biology : CB 2018 Sep 10;28(17):2685-2696.e4
Local activation of mammalian separase in interphase promotes double-strand break repair and prevents oncogenic transformation.
Hellmuth S, Gutiérrez-Caballero C, Llano E, Pendás AM, Stemmann O
The EMBO journal 2018 Nov 15;37(22)
The EMBO journal 2018 Nov 15;37(22)
The ESCRT protein Chmp4c regulates mitotic spindle checkpoint signaling.
Petsalaki E, Dandoulaki M, Zachos G
The Journal of cell biology 2018 Mar 5;217(3):861-876
The Journal of cell biology 2018 Mar 5;217(3):861-876
Studying meiotic cohesin in somatic cells reveals that Rec8-containing cohesin requires Stag3 to function and is regulated by Wapl and sororin.
Wolf PG, Cuba Ramos A, Kenzel J, Neumann B, Stemmann O
Journal of cell science 2018 Jun 11;131(11)
Journal of cell science 2018 Jun 11;131(11)
Chmp4c is required for stable kinetochore-microtubule attachments.
Petsalaki E, Dandoulaki M, Zachos G
Chromosoma 2018 Dec;127(4):461-473
Chromosoma 2018 Dec;127(4):461-473
Role of Kif15 and its novel mitotic partner KBP in K-fiber dynamics and chromosome alignment.
Brouwers N, Mallol Martinez N, Vernos I
PloS one 2017;12(4):e0174819
PloS one 2017;12(4):e0174819
The E2F activators control multiple mitotic regulators and maintain genomic integrity through Sgo1 and BubR1.
Lee M, Rivera-Rivera Y, Moreno CS, Saavedra HI
Oncotarget 2017 Sep 29;8(44):77649-77672
Oncotarget 2017 Sep 29;8(44):77649-77672
SGT1-HSP90 complex is required for CENP-A deposition at centromeres.
Niikura Y, Kitagawa R, Ogi H, Kitagawa K
Cell cycle (Georgetown, Tex.) 2017 Sep 17;16(18):1683-1694
Cell cycle (Georgetown, Tex.) 2017 Sep 17;16(18):1683-1694
The tobacco carcinogen NNK disturbs mitotic chromosome alignment by interrupting p53 targeting to the centrosome.
Park JE, Jang YL, Jang CY
Toxicology letters 2017 Nov 5;281:110-118
Toxicology letters 2017 Nov 5;281:110-118
Mechanism of Ska Recruitment by Ndc80 Complexes to Kinetochores.
Janczyk PŁ, Skorupka KA, Tooley JG, Matson DR, Kestner CA, West T, Pornillos O, Stukenberg PT
Developmental cell 2017 May 22;41(4):438-449.e4
Developmental cell 2017 May 22;41(4):438-449.e4
Spindle assembly checkpoint signaling and sister chromatid cohesion are disrupted by HPV E6-mediated transformation.
Shirnekhi HK, Kelley EP, DeLuca JG, Herman JA
Molecular biology of the cell 2017 Jul 15;28(15):2035-2041
Molecular biology of the cell 2017 Jul 15;28(15):2035-2041
Measuring Kinetochore-Microtubule Attachment Stability in Cultured Cells.
DeLuca KF, Herman JA, DeLuca JG
Methods in molecular biology (Clifton, N.J.) 2016;1413:147-68
Methods in molecular biology (Clifton, N.J.) 2016;1413:147-68
The Ska complex promotes Aurora B activity to ensure chromosome biorientation.
Redli PM, Gasic I, Meraldi P, Nigg EA, Santamaria A
The Journal of cell biology 2016 Oct 10;215(1):77-93
The Journal of cell biology 2016 Oct 10;215(1):77-93
Structure of the MIS12 Complex and Molecular Basis of Its Interaction with CENP-C at Human Kinetochores.
Petrovic A, Keller J, Liu Y, Overlack K, John J, Dimitrova YN, Jenni S, van Gerwen S, Stege P, Wohlgemuth S, Rombaut P, Herzog F, Harrison SC, Vetter IR, Musacchio A
Cell 2016 Nov 3;167(4):1028-1040.e15
Cell 2016 Nov 3;167(4):1028-1040.e15
Preclinical activity of MBM-5 in gastrointestinal cancer by inhibiting NEK2 kinase activity.
Fang Y, Kong Y, Xi J, Zhu M, Zhu T, Jiang T, Hu W, Ma M, Zhang X
Oncotarget 2016 Nov 29;7(48):79327-79341
Oncotarget 2016 Nov 29;7(48):79327-79341
Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore targeting of Mad1-Mad2.
Zhou H, Wang T, Zheng T, Teng J, Chen J
Nature communications 2016 Jan 8;7:10151
Nature communications 2016 Jan 8;7:10151
Molecular basis of outer kinetochore assembly on CENP-T.
Huis In 't Veld PJ, Jeganathan S, Petrovic A, Singh P, John J, Krenn V, Weissmann F, Bange T, Musacchio A
eLife 2016 Dec 24;5
eLife 2016 Dec 24;5
Phosphorylation of Astrin Regulates Its Kinetochore Function.
Chung HJ, Park JE, Lee NS, Kim H, Jang CY
The Journal of biological chemistry 2016 Aug 19;291(34):17579-92
The Journal of biological chemistry 2016 Aug 19;291(34):17579-92
Mitotic Protein CSPP1 Interacts with CENP-H Protein to Coordinate Accurate Chromosome Oscillation in Mitosis.
Zhu L, Wang Z, Wang W, Wang C, Hua S, Su Z, Brako L, Garcia-Barrio M, Ye M, Wei X, Zou H, Ding X, Liu L, Liu X, Yao X
The Journal of biological chemistry 2015 Nov 6;290(45):27053-66
The Journal of biological chemistry 2015 Nov 6;290(45):27053-66
Requirement for PLK1 kinase activity in the maintenance of a robust spindle assembly checkpoint.
O'Connor A, Maffini S, Rainey MD, Kaczmarczyk A, Gaboriau D, Musacchio A, Santocanale C
Biology open 2015 Dec 18;5(1):11-9
Biology open 2015 Dec 18;5(1):11-9
Stable kinetochore-microtubule attachment is sufficient to silence the spindle assembly checkpoint in human cells.
Tauchman EC, Boehm FJ, DeLuca JG
Nature communications 2015 Dec 1;6:10036
Nature communications 2015 Dec 1;6:10036
CENP-W plays a role in maintaining bipolar spindle structure.
Kaczmarczyk A, Sullivan KF
PloS one 2014;9(10):e106464
PloS one 2014;9(10):e106464
Inhibition of Hec1 as a novel approach for treatment of primary liver cancer.
Huang LY, Chang CC, Lee YS, Huang JJ, Chuang SH, Chang JM, Kao KJ, Lau GM, Tsai PY, Liu CW, Lin HS, Gish RG, Lau JY
Cancer chemotherapy and pharmacology 2014 Sep;74(3):511-20
Cancer chemotherapy and pharmacology 2014 Sep;74(3):511-20
PP2A-B56 opposes Mps1 phosphorylation of Knl1 and thereby promotes spindle assembly checkpoint silencing.
Espert A, Uluocak P, Bastos RN, Mangat D, Graab P, Gruneberg U
The Journal of cell biology 2014 Sep 29;206(7):833-42
The Journal of cell biology 2014 Sep 29;206(7):833-42
CENP-I and Aurora B act as a molecular switch that ties RZZ/Mad1 recruitment to kinetochore attachment status.
Matson DR, Stukenberg PT
The Journal of cell biology 2014 May 26;205(4):541-54
The Journal of cell biology 2014 May 26;205(4):541-54
Chk2 prevents mitotic exit when the majority of kinetochores are unattached.
Petsalaki E, Zachos G
The Journal of cell biology 2014 May 12;205(3):339-56
The Journal of cell biology 2014 May 12;205(3):339-56
Activity of a novel Hec1-targeted anticancer compound against breast cancer cell lines in vitro and in vivo.
Huang LY, Chang CC, Lee YS, Chang JM, Huang JJ, Chuang SH, Kao KJ, Lau GM, Tsai PY, Liu CW, Lin HS, Lau JY
Molecular cancer therapeutics 2014 Jun;13(6):1419-30
Molecular cancer therapeutics 2014 Jun;13(6):1419-30
The pseudo GTPase CENP-M drives human kinetochore assembly.
Basilico F, Maffini S, Weir JR, Prumbaum D, Rojas AM, Zimniak T, De Antoni A, Jeganathan S, Voss B, van Gerwen S, Krenn V, Massimiliano L, Valencia A, Vetter IR, Herzog F, Raunser S, Pasqualato S, Musacchio A
eLife 2014 Jul 8;3:e02978
eLife 2014 Jul 8;3:e02978
Pre-anaphase chromosome oscillations are regulated by the antagonistic activities of Cdk1 and PP1 on Kif18A.
Häfner J, Mayr MI, Möckel MM, Mayer TU
Nature communications 2014 Jul 22;5:4397
Nature communications 2014 Jul 22;5:4397
Prophase pathway-dependent removal of cohesin from human chromosomes requires opening of the Smc3-Scc1 gate.
Buheitel J, Stemmann O
The EMBO journal 2013 Mar 6;32(5):666-76
The EMBO journal 2013 Mar 6;32(5):666-76
KNL1 facilitates phosphorylation of outer kinetochore proteins by promoting Aurora B kinase activity.
Caldas GV, DeLuca KF, DeLuca JG
The Journal of cell biology 2013 Dec 23;203(6):957-69
The Journal of cell biology 2013 Dec 23;203(6):957-69
Ubiquitylation-dependent localization of PLK1 in mitosis.
Beck J, Maerki S, Posch M, Metzger T, Persaud A, Scheel H, Hofmann K, Rotin D, Pedrioli P, Swedlow JR, Peter M, Sumara I
Nature cell biology 2013 Apr;15(4):430-9
Nature cell biology 2013 Apr;15(4):430-9
A small-molecule inhibitor of Haspin alters the kinetochore functions of Aurora B.
De Antoni A, Maffini S, Knapp S, Musacchio A, Santaguida S
The Journal of cell biology 2012 Oct 15;199(2):269-84
The Journal of cell biology 2012 Oct 15;199(2):269-84
Subdiffraction imaging of centrosomes reveals higher-order organizational features of pericentriolar material.
Lawo S, Hasegan M, Gupta GD, Pelletier L
Nature cell biology 2012 Nov;14(11):1148-58
Nature cell biology 2012 Nov;14(11):1148-58
Aurora B controls kinetochore-microtubule attachments by inhibiting Ska complex-KMN network interaction.
Chan YW, Jeyaprakash AA, Nigg EA, Santamaria A
The Journal of cell biology 2012 Mar 5;196(5):563-71
The Journal of cell biology 2012 Mar 5;196(5):563-71
A conserved role for COMA/CENP-H/I/N kinetochore proteins in the spindle checkpoint.
Matson DR, Demirel PB, Stukenberg PT, Burke DJ
Genes & development 2012 Mar 15;26(6):542-7
Genes & development 2012 Mar 15;26(6):542-7
Structural analysis reveals features of the spindle checkpoint kinase Bub1-kinetochore subunit Knl1 interaction.
Krenn V, Wehenkel A, Li X, Santaguida S, Musacchio A
The Journal of cell biology 2012 Feb 20;196(4):451-67
The Journal of cell biology 2012 Feb 20;196(4):451-67
Phosphorylation of the Ndc80 complex protein, HEC1, by Nek2 kinase modulates chromosome alignment and signaling of the spindle assembly checkpoint.
Wei R, Ngo B, Wu G, Lee WH
Molecular biology of the cell 2011 Oct;22(19):3584-94
Molecular biology of the cell 2011 Oct;22(19):3584-94
Small GTPase Rab5 participates in chromosome congression and regulates localization of the centromere-associated protein CENP-F to kinetochores.
Serio G, Margaria V, Jensen S, Oldani A, Bartek J, Bussolino F, Lanzetti L
Proceedings of the National Academy of Sciences of the United States of America 2011 Oct 18;108(42):17337-42
Proceedings of the National Academy of Sciences of the United States of America 2011 Oct 18;108(42):17337-42
Direct binding of Cenp-C to the Mis12 complex joins the inner and outer kinetochore.
Screpanti E, De Antoni A, Alushin GM, Petrovic A, Melis T, Nogales E, Musacchio A
Current biology : CB 2011 Mar 8;21(5):391-8
Current biology : CB 2011 Mar 8;21(5):391-8
The NDC80 complex proteins Nuf2 and Hec1 make distinct contributions to kinetochore-microtubule attachment in mitosis.
Sundin LJ, Guimaraes GJ, Deluca JG
Molecular biology of the cell 2011 Mar 15;22(6):759-68
Molecular biology of the cell 2011 Mar 15;22(6):759-68
HJURP is a CENP-A chromatin assembly factor sufficient to form a functional de novo kinetochore.
Barnhart MC, Kuich PH, Stellfox ME, Ward JA, Bassett EA, Black BE, Foltz DR
The Journal of cell biology 2011 Jul 25;194(2):229-43
The Journal of cell biology 2011 Jul 25;194(2):229-43
Evidence that Aurora B is implicated in spindle checkpoint signalling independently of error correction.
Santaguida S, Vernieri C, Villa F, Ciliberto A, Musacchio A
The EMBO journal 2011 Apr 20;30(8):1508-19
The EMBO journal 2011 Apr 20;30(8):1508-19
The Ndc80 complex uses a tripartite attachment point to couple microtubule depolymerization to chromosome movement.
Tooley JG, Miller SA, Stukenberg PT
Molecular biology of the cell 2011 Apr 15;22(8):1217-26
Molecular biology of the cell 2011 Apr 15;22(8):1217-26
The MIS12 complex is a protein interaction hub for outer kinetochore assembly.
Petrovic A, Pasqualato S, Dube P, Krenn V, Santaguida S, Cittaro D, Monzani S, Massimiliano L, Keller J, Tarricone A, Maiolica A, Stark H, Musacchio A
The Journal of cell biology 2010 Sep 6;190(5):835-52
The Journal of cell biology 2010 Sep 6;190(5):835-52
A screen for kinetochore-microtubule interaction inhibitors identifies novel antitubulin compounds.
Screpanti E, Santaguida S, Nguyen T, Silvestri R, Gussio R, Musacchio A, Hamel E, De Wulf P
PloS one 2010 Jul 15;5(7):e11603
PloS one 2010 Jul 15;5(7):e11603
Dissecting the role of MPS1 in chromosome biorientation and the spindle checkpoint through the small molecule inhibitor reversine.
Santaguida S, Tighe A, D'Alise AM, Taylor SS, Musacchio A
The Journal of cell biology 2010 Jul 12;190(1):73-87
The Journal of cell biology 2010 Jul 12;190(1):73-87
Protein phosphatase 6 regulates mitotic spindle formation by controlling the T-loop phosphorylation state of Aurora A bound to its activator TPX2.
Zeng K, Bastos RN, Barr FA, Gruneberg U
The Journal of cell biology 2010 Dec 27;191(7):1315-32
The Journal of cell biology 2010 Dec 27;191(7):1315-32
A mitosis block links active cell cycle with human epidermal differentiation and results in endoreplication.
Zanet J, Freije A, Ruiz M, Coulon V, Sanz JR, Chiesa J, Gandarillas A
PloS one 2010 Dec 20;5(12):e15701
PloS one 2010 Dec 20;5(12):e15701
Hec1 contributes to mitotic centrosomal microtubule growth for proper spindle assembly through interaction with Hice1.
Wu G, Wei R, Cheng E, Ngo B, Lee WH
Molecular biology of the cell 2009 Nov;20(22):4686-95
Molecular biology of the cell 2009 Nov;20(22):4686-95
Mitotic control of kinetochore-associated dynein and spindle orientation by human Spindly.
Chan YW, Fava LL, Uldschmid A, Schmitz MH, Gerlich DW, Nigg EA, Santamaria A
The Journal of cell biology 2009 Jun 1;185(5):859-74
The Journal of cell biology 2009 Jun 1;185(5):859-74
Small molecule targeting the Hec1/Nek2 mitotic pathway suppresses tumor cell growth in culture and in animal.
Wu G, Qiu XL, Zhou L, Zhu J, Chamberlin R, Lau J, Chen PL, Lee WH
Cancer research 2008 Oct 15;68(20):8393-9
Cancer research 2008 Oct 15;68(20):8393-9
Kinetochore attachments require an interaction between unstructured tails on microtubules and Ndc80(Hec1).
Miller SA, Johnson ML, Stukenberg PT
Current biology : CB 2008 Nov 25;18(22):1785-91
Current biology : CB 2008 Nov 25;18(22):1785-91
Aurora A regulates the activity of HURP by controlling the accessibility of its microtubule-binding domain.
Wong J, Lerrigo R, Jang CY, Fang G
Molecular biology of the cell 2008 May;19(5):2083-91
Molecular biology of the cell 2008 May;19(5):2083-91
MCAK-independent functions of ch-Tog/XMAP215 in microtubule plus-end dynamics.
Barr AR, Gergely F
Molecular and cellular biology 2008 Dec;28(23):7199-211
Molecular and cellular biology 2008 Dec;28(23):7199-211
FAM29A promotes microtubule amplification via recruitment of the NEDD1-gamma-tubulin complex to the mitotic spindle.
Zhu H, Coppinger JA, Jang CY, Yates JR 3rd, Fang G
The Journal of cell biology 2008 Dec 1;183(5):835-48
The Journal of cell biology 2008 Dec 1;183(5):835-48
DDA3 recruits microtubule depolymerase Kif2a to spindle poles and controls spindle dynamics and mitotic chromosome movement.
Jang CY, Wong J, Coppinger JA, Seki A, Yates JR 3rd, Fang G
The Journal of cell biology 2008 Apr 21;181(2):255-67
The Journal of cell biology 2008 Apr 21;181(2):255-67
PRP4 is a spindle assembly checkpoint protein required for MPS1, MAD1, and MAD2 localization to the kinetochores.
Montembault E, Dutertre S, Prigent C, Giet R
The Journal of cell biology 2007 Nov 19;179(4):601-9
The Journal of cell biology 2007 Nov 19;179(4):601-9
Centromere targeting of the chromosomal passenger complex requires a ternary subcomplex of Borealin, Survivin, and the N-terminal domain of INCENP.
Klein UR, Nigg EA, Gruneberg U
Molecular biology of the cell 2006 Jun;17(6):2547-58
Molecular biology of the cell 2006 Jun;17(6):2547-58
HURP controls spindle dynamics to promote proper interkinetochore tension and efficient kinetochore capture.
Wong J, Fang G
The Journal of cell biology 2006 Jun 19;173(6):879-91
The Journal of cell biology 2006 Jun 19;173(6):879-91
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- 1/1000 dilution staining ~80 kDa HEC 1 in Hela cell lysate (30mg per well) by Western blot.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEC1 antibody [9G3.23] detects HEC1 protein by western blot analysis. Various whole cell extracts and HeLa nuclear extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HEC1 antibody [9G3.23] (GTX70268) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate (GTX14698).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Hec1 [9G3] antibody detects Hec1 [9G3] protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Hec1 [9G3] antibody (GTX70268) diluted at a dilution of 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HEC1 antibody [9G3.23] (GTX70268) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HEC1 antibody [9G3.23] (GTX70268) diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 £gg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HEC1 antibody [9G3.23] (GTX70268) diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence staining (Olympus FV10i) of HEC1, a kinetochore outer layer marker. U2OS cells were fixed by 4% PFA and costained with Hec1 9G3.23 AB (Red; cat# GTX70268; 1:500 Ab dilution) and alpha-tubulin rabbit polyclonal AB (Green; cat# GTX112141; 1:500 Ab dilution ), a spindle marker. DAPI (blue), chromosomes. Scale bar, 5 um
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed 293T, using HEC1(GTX70268) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.
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- Mouse anti-Hec1 (9G3, cat# GTX 70268) and rabbit anti-Hec1 (phospho Ser165) antibodies were used to co-stain MCF10A cells. Insets show kinetochores of misaligned chromosomes. DAPI staining shows chromatin.
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- Experimental details
- Hec1 antibody [9G3.23] detects Hec1 protein at kinetochore by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Hec1 protein stained by Hec1 antibody [9G3.23] (GTX70268) diluted at 1:500.Red: alpha Tubulin 4a, a cytoskeleton marker, stained by alpha Tubulin 4a antibody (GTX112141) diluted at 1:500.Blue: Hoechst 33342 staining.
