BMS146

antibody from Invitrogen Antibodies

Targeting: LGALS3BP 90K, BTBD17B, CyCAP, gp90, M2BP, MAC-2-BP, TANGO10B

Western blot (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

BMS146

Provider

Invitrogen Antibodies

Product name

90K/Mac-2BP Monoclonal Antibody (SP-2), eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: BMS146 specifically recognizes Mac-2 (galectin-3) mucin-like binding domain of human 90K/Mac-2BP. Mac-2BP is a highly glycosylated protein that plays a major role in cancer research. Elevated levels in sera have been found in many patients suffering from various types of cancer or viral infections. Applications Tested: Immunohistochemistry (Paraffin), Western Blotting. Purity: >95%.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

SP-2

Vial size

100 µg

Concentration

0.1 mg/mL

Storage

4° C

References

Submitted references

Extracellular LGALS3BP regulates neural progenitor position and relates to human cortical complexity.

Kyrousi C, O'Neill AC, Brazovskaja A, He Z, Kielkowski P, Coquand L, Di Giaimo R, D' Andrea P, Belka A, Forero Echeverry A, Mei D, Lenge M, Cruceanu C, Buchsbaum IY, Khattak S, Fabien G, Binder E, Elmslie F, Guerrini R, Baffet AD, Sieber SA, Treutlein B, Robertson SP, Cappello S
Nature communications 2021 Nov 2;12(1):6298

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 1 LGALS3BP expression in human cortical progenitors. a Bulk RNA-sequencing data performed in COs at different stages. LGALS3BP has a peak of expression after apical progenitor marker PAX6 and prior to basal progenitor markers EOMES and HOPX . Data are shown as mean +- SEM, batches = 3, organoids = 3. Statistical analysis was performed using two-way ANOVA followed by Tukey's multiple comparison test, * p < 0.05, ** p < 0.01, **** p < 0.0001. In ( a ), p = 0.0026 in ctrlvsY366Lfs. b Violin plots from scRNA-seq data performed in 60d control COs showing LGALS3BP mRNA expression levels in RGs, IPs, and N, batches = 1, organoids = 2, cells = 9290. c - e In situ hybridization depicting mRNA expression of LGALS3BP in 30d, 50d, and 60d control COs. Arrows show basally located LGALS3B + cells, batches = 1, organoids = 3, ventricles = 3. f - h Micrograph of control CO sections immunostained with specific LGALS3BP antibody. i - q Micrograph of human fetal cortical sections immunostained as depicted in the panels at GW14 ( i - k ) and 20 ( l - q ). Arrows show LGALS3BP+ cells. Scale bar: 30 mum in ( c - h ), 500 mum in ( i - n ), and 100 mum in ( o - q ). See also Supplementary Fig. 1 . Abbreviations: d: days, RG: radial glial cells, IP: intermediate progenitors, N: neurons, GW: gestational week, VZ: ventricular zone, iSVZ: inner subventricular zone, oSVZ: outer subventricular zone, IZ: intermediate zone, CP: cortical plate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 8 LGALS3BP function is mediated by EVs. a Violin plots showing the distribution of gene expression, which are up- or downregulated in mutant cells in scRNA-seq analysis. b Western blot analysis for the expression levels of HA, LGALS3BP, or CD81 antibodies performed in protein extracts isolated from EVs derived from control COs or SH-SY5Y cells upon electroporation of HA, HA-LGALS3BP, HA-E370K, or HA-E294K batches = 2, organoids = 6, cell batches = 3. Source data are provided as a Source Data file. c - m' Immunostaining as indicated in the panels of non-treated control mice sections or after incubation with SH-SY5Y-derived EVs overexpressing HA, HA-LGALS3BP, HA-E370K, or HA-E294K at embryonic day 13 and analyzed 3dpe. h , n Quantification of the percentage of basal Hopx+ cells ( h ) or the distance from the ventricular surface where the first neuronal positive cells are found ( n ) after treatment of control mouse sections with EVs overexpressing HA, HA-LGALS3BP, HA-E370K, or HA-E294K. Data are shown as Z -scores +-SEM. Statistical significance was based on one-way ANOVA and Turkey's multiple comparison test * p < 0.05, ** p < 0.01, *** p < 0.001, n = 3 mice in no EVs, HA EVs, wt-LGALS3BP EVs, n = 4 mice in E370K EVs, n = 5 mice in E294K EVs. In ( h ), p = 0.0002. In ( n ), p = 0.0142. Scale bar: 200 mum. o Summary scheme showing that LGALS3BP is secreted via EVs protein, which upon secretion modulates the extracellular space and promotes changes in the distribution of th