TA503454

antibody from OriGene

Targeting: LGALS3BP 90K, BTBD17B, CyCAP, gp90, M2BP, MAC-2-BP, TANGO10B

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA503454

Provider

OriGene

Proper citation

OriGene Cat#TA503454, RRID:AB_11129609

Product name

LGALS3BP mouse monoclonal antibody, clone OTI1F8 (formerly 1F8)

Antibody type

Monoclonal

Description

LGALS3BP mouse monoclonal antibody, clone OTI1F8 (formerly 1F8)

Host

Mouse

Conjugate

Unconjugated

Epitope

LGALS3BP

Isotype

IgG

Antibody clone number

OTI1F8

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGALS3BP (RC204918, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGALS3BP.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-LGALS3BP monoclonal antibody.(1:200)

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-LGALS3BP mouse monoclonal antibody (TA503454) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LGALS3BP(RC204918).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC204918 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LGALS3BP antibody(TA503454), and then analyzed by flow cytometry.

Validation comment

FC