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LearnAF2226
antibody from R&D Systems
Targeting: LGALS3BP
90K, BTBD17B, CyCAP, gp90, M2BP, MAC-2-BP, TANGO10B
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- AF2226 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Galectin-3BP/MAC-2BP Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Galectin-3BP/MAC-2BP in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant mouse Galectin-3BP/MAC-2BP is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q08380- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Lectin, Galactoside-Binding Soluble 3 Binding Protein Promotes 17-N-Allylamino-17-demethoxygeldanamycin Resistance through PI3K/Akt Pathway in Lung Cancer Cell Line.
Extracellular matrix proteomics identifies molecular signature of symptomatic carotid plaques.
M2BP inhibits HIV-1 virion production in a vimentin filaments-dependent manner.
A galectin-3-dependent pathway upregulates interleukin-6 in the microenvironment of human neuroblastoma.
Human galectin 3 binding protein interacts with recombinant adeno-associated virus type 6.
Diverse functions of reactive cysteines facilitate unique biosynthetic processes of aggregate-prone interleukin-31.
Identification of galectin-3-binding protein as a factor secreted by tumor cells that stimulates interleukin-6 expression in the bone marrow stroma.
Woo JK, Jang JE, Kang JH, Seong JK, Yoon YS, Kim HC, Lee SJ, Oh SH
Molecular cancer therapeutics 2017 Jul;16(7):1355-1365
Molecular cancer therapeutics 2017 Jul;16(7):1355-1365
Extracellular matrix proteomics identifies molecular signature of symptomatic carotid plaques.
Langley SR, Willeit K, Didangelos A, Matic LP, Skroblin P, Barallobre-Barreiro J, Lengquist M, Rungger G, Kapustin A, Kedenko L, Molenaar C, Lu R, Barwari T, Suna G, Yin X, Iglseder B, Paulweber B, Willeit P, Shalhoub J, Pasterkamp G, Davies AH, Monaco C, Hedin U, Shanahan CM, Willeit J, Kiechl S, Mayr M
The Journal of clinical investigation 2017 Apr 3;127(4):1546-1560
The Journal of clinical investigation 2017 Apr 3;127(4):1546-1560
M2BP inhibits HIV-1 virion production in a vimentin filaments-dependent manner.
Wang Q, Zhang X, Han Y, Wang X, Gao G
Scientific reports 2016 Sep 8;6:32736
Scientific reports 2016 Sep 8;6:32736
A galectin-3-dependent pathway upregulates interleukin-6 in the microenvironment of human neuroblastoma.
Silverman AM, Nakata R, Shimada H, Sposto R, DeClerck YA
Cancer research 2012 May 1;72(9):2228-38
Cancer research 2012 May 1;72(9):2228-38
Human galectin 3 binding protein interacts with recombinant adeno-associated virus type 6.
Denard J, Beley C, Kotin R, Lai-Kuen R, Blot S, Leh H, Asokan A, Samulski RJ, Moullier P, Voit T, Garcia L, Svinartchouk F
Journal of virology 2012 Jun;86(12):6620-31
Journal of virology 2012 Jun;86(12):6620-31
Diverse functions of reactive cysteines facilitate unique biosynthetic processes of aggregate-prone interleukin-31.
Shen M, Siu S, Byrd S, Edelmann KH, Patel N, Ketchem RR, Mehlin C, Arnett HA, Hasegawa H
Experimental cell research 2011 Apr 15;317(7):976-93
Experimental cell research 2011 Apr 15;317(7):976-93
Identification of galectin-3-binding protein as a factor secreted by tumor cells that stimulates interleukin-6 expression in the bone marrow stroma.
Fukaya Y, Shimada H, Wang LC, Zandi E, DeClerck YA
The Journal of biological chemistry 2008 Jul 4;283(27):18573-81
The Journal of biological chemistry 2008 Jul 4;283(27):18573-81
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Galectin-3BP/MAC-2BP by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin-3BP/MAC-2BP at approximately 115 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Galectin-3BP/MAC-2BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2226) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Galectin-3BP/MAC-2BP by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human Galectin-3BP/MAC-2BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2226) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Galectin-3BP/MAC-2BP at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Galectin-3BP/MAC-2BP in HeLa Human Cell Line. Galectin-3BP/MAC-2BP was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Galectin-3BP/MAC-2BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2226) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
