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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [1]
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- Product number
- 500-P243-100UG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MIA Polyclonal Antibody, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: MGPMPKLADR KLCADQECSH PISMAVALQD YMAPDCRFLT IHRGQVVYVF SKLKGRGRLF WGGSVQGDYY GDLAARLGYF PSSIVREDQT LKPGKVDVKT DKWDFYCQ. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human MIA. Anti-Human MIA-specific antibody was purified by affinity chromatography employing an immobilized Human MIA matrix. Sandwich ELISA: To detect Human MIA by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human MIA (500-P243Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human MIA. Western Blot: To detect Human MIA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MIA is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100 µg
- Concentration
- 0.1-1.0 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human MIA by Western Blot analysis MIA Polyclonal Antibody (Product # 500-P243-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MIA is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human MIA by Western Blot analysis MIA Polyclonal Antibody (Product # 500-P243-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MIA is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA: To detect Human MIA by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of MIA Polyclonal Antibody (Product # 500-P243-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech MIA Polyclonal Antibody, Biotin (Product # 500-P243BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human MIA.
