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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- HPA040232 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA040232, RRID:AB_2676894
- Product name
- Anti-FOXO4
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human FOXO4, Gene description: forkhead box O4, Alternative Gene Names: AFX1, MLLT7, Validated applications: IHC, ChIP, Uniprot ID: P98177, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Targeted Apoptosis of Senescent Cells Restores Tissue Homeostasis in Response to Chemotoxicity and Aging
Baar M, Brandt R, Putavet D, Klein J, Derks K, Bourgeois B, Stryeck S, Rijksen Y, van Willigenburg H, Feijtel D, van der Pluijm I, Essers J, van Cappellen W, van IJcken W, Houtsmuller A, Pothof J, de Bruin R, Madl T, Hoeijmakers J, Campisi J, de Keizer P
Cell 2017;169(1):132-147.e16
Cell 2017;169(1):132-147.e16
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Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human placenta and liver tissues using HPA040232 antibody. Corresponding FOXO4 RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- ChIP-Exo-Seq composite graph for Anti-FOXO4 (HPA040232, Lot 000028494) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.
