701848

antibody from Invitrogen Antibodies

Targeting: FOXO4 AFX1, MLLT7

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

701848

Provider

Invitrogen Antibodies

Product name

Phospho-FOXO4 (Thr28) Recombinant Rabbit Monoclonal Antibody (12H2L20)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with Monkey, Goat, Pig, Bovine, Dog, Rat and Mouse.

Antibody clone number

12H2L20

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A) Western blot analysis was performed on whole cell extracts (30 µg lysate) of A431 (Lane 1), A431 treated with EGF (200 ng/mL/10 minutes) (Lane 2), A431 treated with PDGF for (200 ng/mL/10 minutes) (Lane 3). The blots were probed with Anti-FOXO4 (pT28) Recombinant Rabbit Monoclonal Antibody (Product # 701848, 0.5-1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 55 kDa band corresponding to FOXO4 (pT28) was observed. B) To confirm the specificity of Anti-FOXO4 (pT28) Recombinant Rabbit Polyclonal Antibody, competition was performed with the phosphopeptide (10 µg/mL) as shown in the corresponding blot on the right. The peptide competes with the antibody and prevents it from binding to the target protein. Known quantity of protein samples were electrophoresed using Novex®NuPAGE®4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002), and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).