711281

antibody from Invitrogen Antibodies

Targeting: FOXO4 AFX1, MLLT7

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

711281

Provider

Invitrogen Antibodies

Product name

Phospho-FOXO4 (Ser197) Recombinant Polyclonal Antibody (12HCLC)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with Monkey, Cat and Dog

Antibody clone number

12HCLC

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on Whole cell extracts (30 µg lysate) of HEK-293 (1), HEK-293 treated with Insulin (Serum starved for overnight, 100nM for 30mins) (2), HeLa (3) and HeLa treated with Insulin (Serum starved for overnight, 100nM for 30mins) (4). The blots were probed with Anti-FOXO4 (pS 197) Recombinant Rabbit Polyclonal Antibody (Product # 711281, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 48 kDa band corresponding to FOXO4 (pS 197) was observed. To confirm the specificity of Anti-FOXO4 (pS 197) Recombinant Rabbit Polyclonal Antibody, competition was performed with phosphopeptide (10 µg/mL) as shown in the corresponding blot on the right. The phosphopeptide competes with the antibody and prevents it from binding to the target protein. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product #

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on Whole cell extracts (30 µg lysate) of HEK-293 (1), HEK-293 treated with Insulin (Serum starved for overnight, 100nM for 30mins) (2), HeLa (3) and HeLa treated with Insulin (Serum starved for overnight, 100nM for 30mins) (4). The blots were probed with Anti-FOXO4 (pS 197) Recombinant Rabbit Polyclonal Antibody (Product # 711281, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 48 kDa band corresponding to FOXO4 (pS 197) was observed. To confirm the specificity of Anti-FOXO4 (pS 197) Recombinant Rabbit Polyclonal Antibody, competition was performed with phosphopeptide (10 µg/mL) as shown in the corresponding blot on the right. The phosphopeptide competes with the antibody and prevents it from binding to the target protein. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product #